is sufficient. Other applications, however, demand a quantitative analysis. Real-time PCR can be used for both qualitative and quantitative analysis; choosing the best method for your application requires a broad knowledge of this technology. This section provides an overview of real-time PCR, ...
What is RT-PCR?Paul S. Auerbach
each unique DNA molecule in the library is bound to the surface of a bead or a flow-cell and PCR amplified to create a set of identical clones. In the case of Ion Torrent technology, a process called “templating” is used ...
SNP-based strain detection is relevant in both clinical and agricultural research and has been used to study a range of infectious diseases in both humans and plants Top Using real-time PCR to perform SNP genotyping Real-time PCR enables you to scre...
However, the first genome draft enabled researchers to pick genes a la carte, produce proteins in vitro for immunological studies, and predict drug targets for the treatment of the disease or to be used in PCR diagnostic protocols. Besides, the analysis of the T. cruzi genome is revealing ...
For example, PCR-amplified DNA can be sequenced, visualised through gel electrophoresis, or cloned into a plasmid for further research. PCR is used in a variety of fields in biology and medicine, including molecular biology, medical diagnostics, and even some aspects of ecology. PCR, like DNA ...
Tropical Medicine PML bodies Main Leishmaniasis, a vector-borne disease that is caused by obligate intra-macrophage protozoa, is endemic in large areas of the tropics, subtropics and the Mediterranean basin (Fig. 1). This disease is characterized by both diversity and complexity1: it is caused ...
What will a PCR product that is heterozygous for DS 180 look like on an agarose gel? How is the correct voltage for agarose gel electrophoresis determined? What is gradient gel electrophoresis? Why is a positive control often used in gel electrophoresis?
Genotyping is an umbrella term for several methods used to analyze variations (e.g., SNPs, insertions or deletions) in genomes between individual organisms.
In medicine, any organism that causes disease. In biological terms, a pathogen is a microorganism that has the inherent capacity to cross anatomical barriers and resist host defences that ordinarily restrict most other microorganisms. Symbiont