is sufficient. Other applications, however, demand a quantitative analysis. Real-time PCR can be used for both qualitative and quantitative analysis; choosing the best method for your application requires a broad knowledge of this technology. This section provides an overview of real-time PCR, ...
Why is PCR used in the process of DNA sequencing? What does Taq DNA polymerase do in PCR? How many primers are used for PCR and why? What controls which DNA is amplified in PCR? How do you caluculate the mass of DNA from PCR?
What is PCR and Different Types of PCR An introduction to PCR and some of the most popular variations of this essential molecular biology technique. Learn More Short-Read Sequencing vs. Long-Read Sequencing What is short-read sequencing, how does it differ from long-read sequencing, and how...
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DNA extraction is a critical first step in the experimental workflow of DNA fragment analysis. The overall efficiency, quality, and size of the PCR product can be significantly affected by characteristics of the sample itself and the method chosen for nucleic acid extraction and purificatio...
For ARMS PCR, also called allele-specific PCR, you need to add four different primers to your master mix. The first primer pair is designed to amplify the DNA sequence containing the SNP of interest (red). The two other primers are sequence-specific for the forward strand of the wild ty...
b. The molecular advantages of the column method that involve the use of Qiagen spin columns is that there are many more steps in the purification process that the resulting DNA is often much purer than those of the precipitation method that mainly focuses on yielding a greater amount of DNA...
1) the amount of DNA available for amplification is limited due to small sample size or other factors (e.g., DNA degradation, PCR inhibition), and 2) interpreting the resulting DNA profile may require more considerations than interpreting single-source or mixed DNA profiles generated using higher...
Why is Taq DNA polymerase used for PCR? What is the importance of DNA polymerase? What is the purpose of a DNA stain in gel electrophoresis? What is the difference between RNA extraction and RNA purif...
Cells or tissues are crosslinked to preserve chromatin–protein interactions, and the protein of interest is immunoprecipitated with an antibody. Quantification of the precipitated DNA by PCR or profiling methods indicates enrichments of the protein of interest. Chromatin remodelers Enzymes or complexes ...