PCR is one of the most important scientific advances of the 20th century1 Molecular Polymerase chain reaction (PCR) is an efficient and cost-effective way to copy small specific DNA or RNA sequences. Plants and animals, bacteria and viruses—every organism has its own unique nucleic acid sequen...
The first step in a PCR cycle heats the starting sample to almost boiling. The high temperature causes DNA melting, or denaturation—the process of physically breaking down the hydrogen bonds between the two complementary strands of the DNA double helix. The result is two single strands of DNA...
is sufficient. Other applications, however, demand a quantitative analysis. Real-time PCR can be used for both qualitative and quantitative analysis; choosing the best method for your application requires a broad knowledge of this technology. This section provides an overview of real-time PCR, ...
The real time PCR instrument used for performing amplification must be capable of distinguishing precisely between these fluorescent labels and measuring the signals produced by the amplification of each gene. Typically, the probe for your gene of interest is labeled...
In this article we will discuss what is PCR tests, PCR steps and its used for, how it works, its stages and its limitations of polymerase chain reaction as a process.
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Efficiency of Real-Time PCR What is multiplexing? Taq Talk Video Series Perhaps you've wondered why some master mixes contain UNG and other master mixes contain UDG and thought to yourself, what is the difference? To answer this question, we first need to establish ...
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