Cancer is a genetic disease that develops through an accumulation of genomic changes in healthy cells. Recent technological advances in next-generation sequencing have dramatically transformed modern genetics and significantly expanded our understanding of the molecular mechanisms of cancer. The knowledge ...
1 However, an American Cancer Society (ACS) study published in 2017 reported that although CRC incidence in the United States is declining overall due to improved screening, it is rapidly increasing among young and middleaged adults (<50 years).2 The study found that CRC rates have in...
This is an exciting time to be in cancer medicine. New technologies, such as next-generation sequencing (NGS), have increased our understanding of the molecular aberrations that define cancer. This, in turn, has led to the identification of cancer-specific molecular targets and potential drugs to...
Clinical applications of next-generation sequencing in cancer diagnosis. Pathol. Oncol. Res. 23, 225–234 (2017). Article CAS Google Scholar Brennan, P. & Wild, C. P. Genomics of cancer and a new era for cancer prevention. PLoS Genet. 11, e1005522 (2015). Article Google Scholar ...
CRISPR genome editing knocks out different genes in different cells. Next-generation sequencing is used to determine which genes are disrupted and which are not. Genes for drug resistance or for drug sensitivity can be identified; negative screens determine genes conferring resistance, and positive ...
public health. Perhaps one of the most widely used examples of this is noninvasive prenatal testing, also known by its acronym, NIPT. Since its introduction in 2011, NIPT uses next-generation sequencing to identify fetal chromosomal abnormalities early in pregnancy with a simple maternal blood ...
Next-generation (next-gen) sequencingis acollection of techniquesthat have further enhanced the speed and detail of genetic sequencing. Instead of sequencing an individual’s entire genetic code from scratch every time, next-gen techniques sequence fragments of an individual’s DNA, called “...
This method can accurately diagnose pan cancer microsatellite instability by single molecule reverse probe capture and high-throughput sequencing. According to this study, smMIPs can only accurately identify microsatellite instability in colorectal, prostate and endometrial cancers to determine the presence ...
Novel Transcripts and Fusion Genes: This method is apt in unveiling new transcripts and detecting fusion genes, especially in cancer research. High-quality control measures, such as filtering raw reads and ensuring optimal sequencing depth, are paramount. Additionally, the number of reads is pivotal...
sequencing has enabled the current study where we report targeted sequencing of 980 candidate tumour suppressor genes in 86 ovarian carcinomas, most of which have matched SNP array data enabling the assessment of the importance of LOH in the selection for somatic mutations in ovarian cancer. We...