FACS: Sorting Cells based on Flow Cytometry Data The terms flow cytometry and fluorescence-activated cell sorting (FACS) are often used interchangeably. In practice, there are differences between the two methods. FACS is a derivative of flow cytometry that adds an exceptional degree of functionality...
Flow Cytometry Flow cytometry is a system for sensing individual cells in a physiologic saline solution as they move in a focused liquid stream through a fixed laser beam scattering light and emitting fluorescence that is measured and converted into digitized data. ...
Related to FACS:Flow cytometry Category filter: AcronymDefinition FACSFellow, American College of Surgeons FACSFamily And Consumer Sciences FACSDepartment of Family and Community Services(Australia) FACSFacial Action Coding System FACSFamily & Community Services(various organizations) ...
Intracellular flow cytometryis a powerful technique for the identification of cell types and the analysis of signaling and functional responses within cell lines and heterogeneous cell samples. BD Biosciences provides a variety of reagents, kits andprotocolsto facilitate intracellular flow cytometry. Find ...
Flow cytometer and cell sorter manufacturers have invested considerable resources to design instruments that are the “fastest in the ‘hood” either in terms of cells analyzed per second, or in total throughput. The general idea is the faster you can go, the quicker you can identify rare ...
FACSFlow cytometryFluorescence-activated cell sortingIn vitro disease modelsNeural developmentNeural stem cellsNeuroregenerationNot only have our insights into neural development at a cellular level dramatically increased over the past decade, we are also on the verge of utilizing our knowledge to now ...
N/A: not available; FACS: fluorescent-activated cell sorting; SCLC: small cell lung cancer; NSCLC: non-small cell lung cancer; 2D: two-dimensional culture; 3D: three-dimensional culture. HEA: Human epithelial antigen. References Khoo, B.L.; Grenci, G.; Jing, T.; Lim, Y.B.; Lee, ...
Gene expression was induced with tetracycline (1 mg/L), and cells expressing the EYFP tag were sorted out in a FACS-Diva flow cytometer (BD Biosciences). The transfection efficiency was evaluated in cells expressing LDLR coupled to the EYFP tag and analyzed by flow cytometry. There is no spi...
What is FACS? FACSis an abbreviation for “fluorescence-activated cell sorting”, which is a flow cytometry technique that further adds a degree of functionality. By utilizing highly specific antibodies labeled with fluorescent conjugates (a fluorescent molecule called fluorochrome), FACS analysis allows...
Flow Cytometry vs. FACS The terms flow cytometry and FACS are often used interchangeably, but they are not actually the same things. FACS is a specialized type of flow cytometry that applies fluorescent markers to cells and takes advantage of the single-cell stream to target and isolate cell ...