Gram staining is the technique used to identify Gram-positive and Gram-negative bacteria. In this staining technique, crystal violet is used as a mordant, and alcohol is used as a decolorizing agent. Answer and Explanation: A bacterial smear is prepared to fix the bacteria on the...
Iodine fixes the crystal violet into the cell wall of the bacteria by working as a mordant. A mordant forms a complex that adheres tightly to the cell... Learn more about this topic: Gram Stain | Purpose, Theory & Procedure from
It is another useful and commonly used method for bacterial identification. Gram stainingis the most important type of staining method used in microbiology for bacterial identification. It is a differential staining technique used to differentiate bacteria into two groups; Gram Positive and Gram Negative...
Basicstains, such as methylene blue, Gram safranin, or Gram crystal violet are useful for staining most bacteria. These stains will readily give up a hydroxide ion or accept a hydrogen ion, which leaves the stain positively charged. Which dye is used for staining plant cells? Thefluorescent dy...
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Gram stains (Crystal violet, Gram's iodine, Counterstain (safranin) and a decolorizing agent) Sample - Sample from a patient or culture. (If a sample from a culture is used, it should not have been incubated for long). Water (distilled or tap water) ...
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Gram staining is a microbiological method developed by Hans Christian Gram. It classifies bacteria into two major groups: Gram positives and Gram negatives. Answer and Explanation: The process of gram staining occurs as followed: 1. Crystal violet, a primary ...
You view your Gram stain of the Gram standard and see only purple cells. What may have gone wrong? a. Used iodine as the mordant b. Decolorized with 95% ethanol c. Used crystal violet for the counterstain d. Did ...