Gram staining is the technique used to identify Gram-positive and Gram-negative bacteria. In this staining technique, crystal violet is used as a mordant, and alcohol is used as a decolorizing agent. Answer and Explanation: A bacterial smear is prepared to fix the bacteria on the...
Iodine fixes the crystal violet into the cell wall of the bacteria by working as a mordant. A mordant forms a complex that adheres tightly to the cell... Learn more about this topic: Gram Stain | Purpose, Theory & Procedure from
Basicstains, such as methylene blue, Gram safranin, or Gram crystal violet are useful for staining most bacteria. These stains will readily give up a hydroxide ion or accept a hydrogen ion, which leaves the stain positively charged. Which dye is used for staining plant cells? Thefluorescent dy...
It is another useful and commonly used method for bacterial identification. Gram stainingis the most important type of staining method used in microbiology for bacterial identification. It is a differential staining technique used to differentiate bacteria into two groups; Gram Positive and Gram Negative...
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Among Clostridium, a majority are Gram-positive rods. For instance, C. difficile, associated with gastrointestinal illness, is one of the Gram-positive Clostridium that will appear dark blue/violet having retained the primary stain (crystal violet) in their thick peptidoglycan layer....
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Gram staining is a microbiological method developed by Hans Christian Gram. It classifies bacteria into two major groups: Gram positives and Gram negatives. Answer and Explanation: The process of gram staining occurs as followed: 1. Crystal violet, a primary ...