PCR is one of the most important scientific advances of the 20th century1 Molecular Polymerase chain reaction (PCR) is an efficient and cost-effective way to copy small specific DNA or RNA sequences. Plants and animals, bacteria and viruses—every organism has its own unique nucleic acid sequen...
QSY is a non-fluorescent quencher, however the QSY probes don’t have a MGB moiety. If for example, you are running a 4-plex reaction, two TaqMan assays can be FAM and VIC probes labeled with an MGB-NFQ quencher while the other two assays should have...
PCRtechniqueDNAThe polymerase chain reaction (PCR) is a technique commonly used by molecular biologists to make additional copies of DNA, which may be used for a variety of purposes (see pp. 38-41).Benjamin EvansLaura EmeryBiological sciences review...
How to start an NGS lab Watch this video to learn how easy it is to set up your own NGS lab at your institution. In this video, you will learn: The requirements for laboratory space and environmental conditions to support optimal NGS testing ...
PCR amplification are performed in the same or separate tubes. In the two-step method, RNA is first transcribed into cDNA in a reaction using reverse transcriptase. An aliquot of the resulting cDNA is then used as a template for multiple qPCR reactions. In the one-step method, RT and qPCR...
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您见过拥有120个通道的荧光定量PCR仪吗?您见过可以用U盘运行的荧光定量PCR仪吗?您见过可以通过WiFi 控制的荧光定量PCR仪吗?您见过体重不到2kg的荧光定量PCR仪吗?您见过会说话的荧光定量PCR仪吗?IT-IS MyGo系列荧光定量PCR仪满足你的所有好奇。 MyGo Pro 实时荧光定量PCR仪 ...
The wide range of applications of PCR has led to an ever-growing list of variants of the technique. We will focus on the conventional PCR which is the most basic type of PCR reaction and this question: what are the three basic steps of conventional PCR. Try Conventional PCR in Praxilabs...
Genotyping is the experimental procedure that identifies the differences in DNA sequence among individuals or populations. It is used to understand the connection between genotype (the underlying genetic code) and phenotype (the observable organismal structure). An individual genome is identified as a di...
内参即内源性参照,qPCR实验中用于校正因样本量大小或RNA损失等原因导致的基因表达量的变化。如果把基因比作绳子,基因表达量比作绳子的长度,内参基因就像直尺,以它为参照物,我们能更准确地了解绳子的长度。 图1 内参的归一化作用(图源网络) 理想...