What is reverse transcriptase, and why is it an important tool of recombinant DNA technology? (a) Define recombinant DNA. (b) Explain their role in genetic engineering. What characteristics of plasmids make them good cloning vectors? Answer of the following question. What is a plasmid?
Importantly, when x-gal is catabolized the byproduct, 5-bromo-4-chloro-indoxyl, dimerizes producing an insoluble blue pigment. However, colonies made up of cells that accept a plasmid with the DNA insertion remain white, because the DNA that was inserted into the cloning vector at the MCS...
(1) PCR is a technology that has many useful applications with biotechnology. (a) What are some of those applications? (b) What are the advantages of PCR over gene cloning for generating many copies of a DNA fragment? (2) Humans are almost identical in th ...
Using broad- range primers, such as those that amplify the 16S rRNA gene, a single PCR can be used to detect multiple pathogens simultaneously. DNA microarrays have been used to detect bacteria and viruses24,25 and, because the number of possible hybridizations is now very high, sequencing ...
Degenerate peptides are peptides that are shared by multiple proteins and their presence implicates the presence of all the proteins that share these peptides (Huang et al.). The goal of “Structural Genomics” is the determination of the structure of all human proteins. Structural models of ...
How does the expression zone shift from the developing vascular tissues to the lateral root founder cells in the pericycle? Furthermore, is signal transmission resulting from the interaction of RALF34–THE1 involved in determining the competence of pericycle cells to initiate the formation of a ...
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The challenge with the malaria parasite in general and its AMA1 surface protein in particular is that both exist as multiple strains. Mixing right cocktail of proteins can help build better malaria vaccine Autonomous plasmid replication in Aspergillus nidulans: AMA1 and MATE elements. Cloning and ex...
(BE). A detailed description of the single techniques is summarized in Additional file1. Site-directed nucleases induce double-strand breaks (DSBs) in the DNA which are subsequently repaired by the autochthonous cellular mechanisms. The type of repair can be categorized in three main types [10,...
The discovery of a new hemoprotein from rabbit liver in 1962 is one of the key moments in biology. Today, cytochrome P450 enzymes constitute a protein superfamily superfamily found in all domains of life and have even been