What is the difference between the purpose of DNA cloning and PCR? Do both of them make many copies of the desired gene? What is a better tool to produce insulin - plasmid or PCR? Plasmid: A plasm...
Importantly, when x-gal is catabolized the byproduct, 5-bromo-4-chloro-indoxyl, dimerizes producing an insoluble blue pigment. However, colonies made up of cells that accept a plasmid with the DNA insertion remain white, because the DNA that was inserted into the cloning vector at the MCS...
Discuss how PCR can be used to replicate DNA and why it is such a useful tool. How are primers involved in the replication of DNA? What is the function of the Taq DNA poltmearse in a PCR reaction? (a) How PCR is similar to DNA cloning? (b) How PCR is different from DNA clonin...
Multiple cloning site (MCS) is defined as ___ A. site within the plasmid which contains a site for many restriction enzymes B. site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid C. as the site containing ma...
Gene therapy can be described as a disease treatment that introduces exogenous genetic material such as plasmid DNA, antisense oligonucleotides, mRNA or peptide nucleic acids into cells or tissues with the aim of treating hereditary or acquired genetic disorders. In essence, it is the use of nuclei...
A Brazilian Middle West Perspective First described in 1908 by Adolpho Lutz as "A Pseudococcidic Mycosis Localized in the Mouth and Observed in Brazil" [1], paracoccidioidomycosis is the most prevalent systemic mycosis in South America. This fungal disease was named Brazilian blastomycosis, South ...
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(BE). A detailed description of the single techniques is summarized in Additional file1. Site-directed nucleases induce double-strand breaks (DSBs) in the DNA which are subsequently repaired by the autochthonous cellular mechanisms. The type of repair can be categorized in three main types [10,...
After several months of cDNA cloning and sequencing, Kiki, Laura, and I determined the TRF protein sequence in late 1993. To my disappointment, TRF was not homologous to Rap1. Worse, TRF looked like a transcription factor, with an Myb-type DNA–binding domain at the C terminus and an aci...
KRAS G12C is another significant mutation found in 13% of lung cancers and 3% of colorectal cancer [7], [8]. This mutation, identified as an oncogenic driver of tumorigenesis, has been considered druggable, as the presence of the substituted cysteine provides a target for drug binding [5]...