In Part I the chemistry of enzyme assays is discussed, in Part II the assays used in the context of genetic selection are covered, and in Part III multisubstrate as- says for biochemical characterization of enzymes are discussed. Before even starting into t...
A CDx assay is required when the drug/biologic has a specific genetic or biological target not present in all patients with a particular disease. Companion diagnostic tests can be used to identify patients most likely to respond to a therapeutic drug or biologic, as well as patients at lower ...
This means there is a SNP at this specific position with two possible nucleotide variations: C or A. There are over 660 million SNPs in the human genome, which makes them the most common type of genetic variation in humans. They can explai...
To set up an IFC, first load your samples into the plate. Then, add a different master mix to each assay inlet. As with genotyping by qPCR, every master mix has to contain a primer pair binding to a DNA sequence containing a SNP of interest, as well as probes specific to both the ...
Each B cell expresses a different antibody (the B cell receptor) at its surface as a result of genetic mechanisms including V(D)J recombination, junctional diversification, and somatic hypermutation. When a B cell encounters its specific antigen, it differentiates to form short-lived plasma cells...
Genetic variation can be passed to the next generation and can increase or decrease the fitness of a given species. Genotyping is the experimental procedure that identifies the differences in DNA sequence among individuals or populations. It is used to understand the connection between genotype (the...
(a) What is an example of genetic testing? (b) How it can benefit us? What is the best way to gain experience understanding medical journals? What are IMViC tests and why are they valuable? Explain. Explain why specimens should be taken aseptically, even when nonsterile s...
What is infectious disease control? Is there a test for adenovirus? What is the adenovirus vaccine? How does the Baltimore classification help with virology? What is virus transduction? What does provirus do during HIV? What is the goal of the viral plaque assay?
The evolution of plants to efficiently transport water and assimilates over long distances is a major evolutionary success that facilitated their growth and colonization of land. Vascular tissues, namely xylem and phloem, are characterized by high specia
Visceral leishmaniasis (VL) is a systemic protozoan disease that is transmitted by phlebotomine sandflies. Poor and neglected populations in East Africa and the Indian sub-continent are particularly affected. Early and accurate diagnosis and treatment re