The PCR technique uses (a) heat-resistant DNA polymerase (b) reverse transcriptase (c) DNA ligase (d) restriction enzymes (e) b and c During colony PCR, we often use the Sapphire DNA polymerase. Can we use this same polymerase for seq...
As multiplexing minimizes pipetting errors, you might assume that by using this technique, you guarantee low variation in Ct values between replicates. This is not always the case, as variation can arise from interactions between the complex mix of reagents in the we...
Once the foreign DNA and plasmid DNA has been linked together, what techniques are used to get the vector into the bacterial cells? What does the cutting enzyme do regarding DNA fingerprinting? What is CRISPR-Cas9? How is the technique useful for iPS cell studies?
The PCR is a method for making many copies of ___ DNA. What properties of DNA does PCR take advantage of? Why must post-PCR and pre-PCR sections be seperated in genetics laboratories? What techniques were used in the study of sickle cell anemia-electrophoresis, restr...
In the mid-1970s, Sanger wasn’t alone in the race to sequence DNA; almost in parallel, two American scientists, Maxam and Gilbert, developed a technique in which DNA is chemically treated to break the chain at specific bases. Following electrophoresis...
What is the latest best procedural technique for TAVI (balloon valvuloplasty or not, valve positionning, use of permanent pace maker)Lars Sondergaard
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(a) List the steps in PCR. (b) Give an example of its use. Describe the process of PCR. Briefly describe the three steps used in PCR? What is PCR? Describe the technique in detail. Explain the basic process of PCR. How does PCR work? What are the steps to be taken in the PCR...
Polymerase Chain Reaction (PCR):In short, PCR is the selective replication of DNA in a test tube. All PCR reactions follow the same basic strategy. We start with a piece of double-stranded DNA that we wish to amplify. Two primers of a defined sequence that are complementary to the ...