声明: 本网站大部分资源来源于用户创建编辑，上传，机构合作，自有兼职答题团队，如有侵犯了你的权益，请发送邮箱到feedback@deepthink.net.cn 本网站将在三个工作日内移除相关内容,刷刷题对内容所造成的任何后果不承担法律上的任何义务或责任
At what temperature does DNA elongate? Polymerase Chain Reaction Polymerase Chain Reaction (PCR) is an in vitro technique in biochemistry and molecular biology laboratories in which large amount of deoxyribonucleic acid (DNA) is synthesized. The starting materials for PCR are: (1) the DNA desired ...
During PCR and cycle sequencing, the DNA is first denatured (the double-stranded DNA template becomes single-stranded DNA). A subsequent annealing step allows for hybridization of the oligonucleotide primer close to the sequence of interest. In the extension...
How is PCR used in DNA sequencing? What is a gel electrophoresis chamber? What is the importance of DNA sequencing? Why are there multiple bands in gel electrophoresis? How is DNA sequencing useful? What does DNA ligase do? What is the purpose of DNA helicase?
PCR-grade water: Certified to be free of contaminants, nucleases and inhibitors. dNTPs: Containing equal concentrations of the four nucleotides (dATP, dCTP, dGTP and dTTP), which are the 'building blocks' to create complementary copies of the DNA sequence of interest. ...
Browser In Silico PCR utility at: genome.ucsc.edu/cgi-bin/hgPcr. It is also important to make sure that amplicons are all approximately the same size, and that primer and probe dimers do not form (across all primer pairs). Use ourMultiple Primer Analyzertool ...
The first step in a PCR cycle is to heat the sample to 95°C. What is the purpose of heating the sample?A.To separate the two strands of DNAB.To allow the primer to anneal to the templateC.To produce the optimal temperature for Taq dna polymerase to workD.To produce the optimal ...
PCR: PCR is a Polymerase Chain Reaction. Kary Mullis developed it in 1983. It is a technique used to amplify a specific segment of DNA. The technique mainly involves three steps: denaturation, annealing, and elongation. This technique is commonly used in molecular...
What does DNA polymerase catalyze? What does DNA polymerase do in PCR? What is the substrate of DNA polymerase? What enzyme facilitates hydrogen bonding between nucleotides? What does gel electrophoresis do to DNA? What is Taq DNA polymerase used for?
What is a possible effect of an error during DNA transcription? what is the difference between DNA Polymerase 1 and 3? What does DNA polymerase do in PCR? What is the transcription process in DNA? What errors can occur? What kind of protein is DNA polymerase?