During PCR and cycle sequencing, the DNA is first denatured (the double-stranded DNA template becomes single-stranded DNA). A subsequent annealing step allows for hybridization of the oligonucleotide primer close to the sequence of interest. In the extension...
When does DNA replication occur? Where does DNA replication occur? Learn about DNA polymerase and enzymes, DNA replication steps, and DNA replication process. Related to this Question What is the full form of DNA and RNA? What is the full form of DNA, the blueprint of life?
Which RNA base pairs with the adenine in DNA? How does DNA polymerase add nucleotides? What is the structural difference between the two pentose sugars that comprise DNA & RNA nucleotides? Explain what is the role of nucleotides in energy production? (a biology question) ...
What distinguishes the language of biology from that of physics is its free and full use of the because of reason. Where the inanimate world lends itself in some regards to application of a “deadened,” skeletal language — a language that perhaps too easily invites us to think in terms ...
What does the transcription of cells mean in cellular biology? What are the components of the pre-replication complex found in eukaryotes? What control of gene expression begins when processed mRNA reaches the cytoplasm and before there is a protein product?
The use of a strand-displacing DNA polymerase in LAMP test also precludes the use of hydrolysis probes, e.g. TaqMan probes, which rely upon the 5'-3' exonuclease activity of Taq polymerase. An alternative real-time multiplexing approach based on fluorescence quenchers has been reported. ...
does residual HIV transcription per se mean residual virus replication (discussed in detail in [10]). The debate about the possibility of residual HIV replication despite ART has been ongoing for a long time [43], but recently gained some renewed momentum with the publication of an intensive ...
Cell-associated (CA) HIV RNA has received much attention in recent years as a surrogate measure of the efficiency of HIV latency reversion and because it may provide an estimate of the viral reservoir size. This review provides an update on some recent i
Why does DNA polymerase always act 5' to 3'? What are the functions of introns? What are three different types of cofactors and their functions? What are the enzymes' or proteins' role in a visceral smooth muscle cells function? If enzymes speed up a chemical rxn, how does it lower the...
What are the proteins involved in homologous recombination? Describe DNA replication. What does it mean that DNA bases are complementary? Relating to biology, explain the term 'replication origin'. Define what is a genome and briefly describe what is it made of. What is the significance of mito...