Recent development of knockout mice with targeted deletion of specific genes encoding various red cell membrane proteins has added valuable armamentarium to red cell membrane structure鈥揻unction studies. In this chapter we will summarize the various recent developments regarding the structure and function...
The p53 tumor suppressor gene (TP53 in humans or Trp53 in mice) is critical for inhibiting tumor development in many tissues. This is evident in breast epithelium from the high frequency of mutations in TP53 in sporadic human breast cancers [1]. Consiste
In mammalian cells, ACs are divided into two superfamilies: plasma membrane-bound adenylyl cyclases (tmACs) and soluble (intracellular) adenylyl cyclases (sACs) [29,30]. There are nine genes encoding tmACs and a single gene encoding the isoforms of sAC [31]. TmACs are ubiquitous and are ...
Recently, CRISPR was used to generate triple-knockout, ‘off-the-shelf’ CAR T cells which are hypoimmunogenic, meaning that they can be used to treat any patient - a massive breakthrough for T cell immunotherapy. CRISPR-Cas9 edited iPSCs can also be used to generate organoids - tiny, ...
(GRP) on vascular inflammationSignal transduction - Heart323A new synthetic peptide regulates hypertrophy in vitro through means of the inhibition of nfkb324Inducible fibroblast-specific knockout of p38 alpha map kinase is cardioprotective in a mouse model of isoproterenol-induced cardiac hypertrophy325...
A mechanism to replace faulty genes is called \rule{2cm}{0.4pt}. a) gene therapy b) gene knockout therapy c) DNA microarrays d) PCR Describe the Sanger method of DNA sequencing. What is the function of DNA polymerase in PCR? a. raw material for new DNA b. atta...
MHC genes are highly polymorphic in humans and mammals. Furthermore, it has significant geographical and ethnic differences in the distribution of its dominant genotypes. In addition, most of the common international MHC transgenic mouse models are HLA-I or HLA-II single-transgenic mice, which stil...
the low efficiency of gene vector transfection, improper gene integration, and immune response caused by foreign genes have restricted breakthroughs in gene therapy. There are still some key technical problems to be solved in gene therapy: for example, how to efficiently introduce targeting gene into...
regulatory elements likely to control genes important for cell type specification. This is a preview of subscription content,access via your institution Access options Subscription info for Chinese customers We have a dedicated website for our Chinese customers. Please go tonaturechina.comto subscribe ...
KO knock-out LDLR low-density lipoprotein receptor LPS lipopolysaccharides LXR liver X receptor MAPT microtubule-associated protein tau MAPT-Tg transgenic mouse with human tau MAPT/APOE-Tg transgenic mouse with human tau and human APOE MCI mild cognitive impairment M months m- mouse NFT neurofibrillar...