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In the final step, the primers are extended by DNA polymerase, resulting in two copies of the original DNA strand. The main applications of PCR include genetic testing and detection of pathogenic DNA. RT-PCR In RT-PCR (reverse transcription PCR), RNA is used as the sta...
Describe the polymerase chain reaction (PCR). What are some advantages and limitations of this procedure? Why doesn't RNA polymerase need a primer? How can a DNA polymerase use a protein as a primer for replication? What is the significance of the use of Taq polymerase? Explain when...
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What are the applications of capillary electrophoresis? NGS Education for Sanger Sequencing Users Sanger sequencing is a method that yields information about the identity and order of the four nucleotide bases in a segment of DNA. Also known also as th...
Finally, because real-time qPCR reactions are run and data are evaluated in a unified, closed-tube qPCR system, opportunities for contamination are reduced and the need for postamplification manipulation is eliminated in qPCR analysis. Applications of Real-Time PCR/qPCR Assays Real-time PCR/qPCR ...
During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system. Data...
存在主义心理学家欧文·亚隆提出了波动影响这一概念，认为我们每个人都可以形成中心影响力，就像激荡的池塘涟漪一样，将这种影响传递给周围的每一个人。这种影响只有在我们有权利、有地位、有声望的时候才会产生。（ ）
Background [1-3] Long-chain fatty acid acyl-CoA hydrolase antibodies are a type of polyclonal antibody that can specifically bind to long-chain fatty..
What Are the Concepts Related to PCEP? How Does PCEP Work? PCEP Applications Why Do We Need PCEP? Path computationcanbe a complex task to complete, especially on large multi-domain networks. In some scenarios, it requires a special computation component to be deployed on the controller, and ...