Fluorescent western blotting for dementia research and beyondIntroduction to ProteintechProteintech has been the original manufacturer of antibodies, ELISA kits, and proteins since 2002. We have antibodies agai
Fluorescent western botting provides accurate, quantitative, stable signals, and the ability to clearly evaluate multiple protein targets on a single blot. We offer optimized reagents, buffers and fluorescent conjugated antibodies so you can get the m...
Western or immunoblotting analysis of protein expression in cells and tissues has been the major analytical tool for assessing molecular biological functions in basic cell biological research, pathology and drug discovery and development. With the rapid growth of our understanding of gene and protein ex...
Fluorescent Western Blotting: High Sensitivity Detection of Multiple Targetsfluorescencemultiplexed protein detectionwestern blotWestern blotting with fluorescence detection offers the possibility of detecting multiple targets simultaneously on a single blot. Primary antibodies are increasingly available from multiple...
Use StarBright and hFAB fluorescent antibodies for high-sensitivity multiplex detection in western blotting. One-step detection of actin, tubulin, and GAPDH.
antibodies and the western blotting membrane. This results in less background and brighter target signal when using the TrueBlack®Fluorescent Western Blot Blocking Buffer Kit compared to the standard antibody diluent and blocking buffers. If using this kit, please use the kit-specific protocol on...
Fluorescent Western standardization blot Blotting papers ELISA SARS-CoV-2 Proteins and Kits AdvanBlock-EIA blocking solution AdvanBlock-PF blocking solution 10X EIA Coating Buffer ELISABright Chemiluminescent substrate HRP-conjugated secondary antibodies ...
Techniques for localizing contractile proteins with fluorescent antibodies. Curr. Top. Dev. Biol. 14, 271–296 (1980). Article CAS PubMed Google Scholar Kuzmin, A. N., Pliss, A. & Prasad, P. N. Changes in biomolecular profile in a single nucleolus during cell fixation. Anal. Chem. 86...
Advantages of fluorescent probes for western blots Qdot® and Alexa Fluor® secondary antibody conjugates are revolutionizing western blot detection. When paired with a fluorescence imager, these detection reagents provide sensitive and quantit...
Place the blot in diluted fluorescent dye-labeled secondary antibody solution and incubate for 1 hour with gentle agitation. Wash the blot with wash buffer 3–5 times for 5 minutes each. Place the blot onto a piece of clean filter paper to dry. ...