be problematic for immunoprecipitation (IP) and pull-down assays prior to western blot. When it’s crucial to preserve protein-protein interactions or to minimize denaturation, you should use a buffer without ionic detergents (eg SDS) and ideally without non-ionic detergents (eg Triton X-100). ...
Proteintech生产的Western Blot湿法转膜液适用于Western Blot湿法电转膜。本产品是一种安全无毒的转膜液,不含甲醇及其它有毒试剂。本品可配制Western湿法转膜液1 升,方法非常便捷。 组分1L Transfer Buffer干粉 保存条件 4℃保存,1 年有效。 使用方法 1.取本品倒入到一洁净的烧杯内,加入蒸馏水到约 700mL(或 500mL...
Recipe for Western Blot resolving gel solution Semi-dry transferWet transfer Transfer time<1 h1 h to overnight Buffer volumes requiredSmallLarge Transfer efficiencyLowHigh Large proteinsUnsuitableSuitable Small proteinsSuitableSuitable Bands on membraneTendency to produce indistinct bandsClear, sharp bands ...
Universal Eectrophoresis and Transfer buffer (for Western blot) Western blot电泳电转通用缓冲液 20329ES03 1 L 室温 15.00 产品描述 Western blot实验首先需要进行电泳分离目的蛋白,然后将分开的蛋白从凝胶转移到固相基质如硝化纤维素膜或PVDF膜上,从而进行随后的免疫印迹检测。Tris-甘氨酸是最通用的电泳和转膜缓冲...
英文名称:Western wet membrane fluid 保质期:长期 个月 保存条件:冷藏 Western湿转转膜液说明书 产地:国产|进口 英文名:Western wet membrane fluid 编号:RFT080 规格:10×1L 本Western转膜液是一种安全无毒的转膜液,没有使用剧毒的甲醇,也没有使用其它的有毒试剂。本Western转膜液配制便捷,无需调节pH值,用于Wes...
Western Blot layers courtesy of Cusabio. Click to enlarge. Assemble the transfer device, and transfer proteins to nitrocellulose or PVDF membrane. We recommend a 0.22 μm membrane for proteins with weights lower than 20 kD. There are two transfer methods: wet transfer or semi-dry-transfer. Both...
蛋白免疫印迹实验,或Western Blot(又称为免疫印迹,因为使用了抗体来特异性检测其抗原)于1979年由 Towbin 等人提出,现在已是常规的蛋白分析技术。抗体-抗原相互作用的特异性实现了复杂蛋白质混合物中的靶蛋白识别。通过蛋白免疫印迹可获得关于目标蛋白质的定性和...
售价 ￥100.00 0销量 手机购买 商品品牌:Proteintech 商品型号:PR20010 规格 1 L 数量 (库存1000包) 详情 评价(0) 没有评论数据 心中有疑问就问问其他同学吧~ 我要提问 ￥350.00 Goat anti-Rabbit IgG ￥1400.00-29590.00 TH5427 ￥100.00 Goat Serum ...
Western blot transfer apparatus.Schematic showing the assembly of a typical western blot apparatus with the position of the gel, transfer membrane, and direction of protein in relation to the electrode position. Although the image depict...
1. Wet Transfer In wet transfer a similar gel tank is used to SDS-PAGE in that the gel and membrane stand vertically, but this time with the current moving across instead of down the tank. The tank is filled with transfer buffer, which is similar to running buffer as it allows for the...