2.5. Western Blot Analysis Total protein extracts from liver were obtained by the Helenius method [22], and protein concentrations were determined by the Bradford method [23]. Thirty micrograms of protein extracts were subjected to SDS-polyacrylamide gel electrophoresis. The protein fractions were ...
Analysis of mouse myogenic cells by the Northern blot assay showed that Dp71 is expressed in myoblasts, is downregulated during in vitro myogenesis and is undetected in differentiated muscle cell cultures [6]. However, reverse transcription (RT)-PCR amplification identified Dp71 transcript in mouse ...