For those proteins that have not been tested with natural samples, a protocol and troubleshooting guide is available for Western blot optimization. Please note that further optimization such as antibody concentration and buffers may be required. Please read the following Western blot protocol in its ...
Optional: To visualize the molecular weight markers in addition to the protein of interest, add 1 μg/mL Blue Marker Antibody to the Primary Antibody Solution. This antibody does not cross react with protein lysates and will bind specifically to the blue dye of each molecular weight marker. ...
Contamination can be transferred to the blots from electrophoresis and related equipment used in blot preparation. Post-antibody washes may not have been performed for a sufficient period of time or were not performed in a high enough volume. The blocking and incubation agents used were not ...
Rinse the blot in PBS for approximately 5 mins. Block the membrane using 1% casein in PBS (may use 1% BSA or 5% non-fat dry milk) for 1 hour at room temperature. Antibody incubation Dilute the primary antibody in blocking buffer and incubate 1-3 hours at room temperature, or overnight...
Western Blot protocol for Nanog Antibody (NBP2-13177)***Protocols, Publications, Related Products, Reviews and more:www.novusbio.com/NBP2-13177 Orders:***Support:www.novusbio.com Web:NBP2-13177 Protocol Nanog Antibody: https://www.novusbio.com/products/nanog-antibody_nbp2-13177 Western Blot ...
For western blots, incubate membrane with diluted primary antibody in either 5% w/v BSA or nonfat dry milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Reasons to use the Cell Signaling Technology western blotting protocol ...
Incubate the membrane with a suitable HRP-conjugated secondary antibody (recognizing the host species of the primary antibody), diluted according to the instructions. Incubate for 1 hour with constant rocking. Wash membrane three times with 1x TBST for 10 minutes each. Tip: Do not let the membr...
NOTES:-Use treated slides (e.g. HistoBond) to assure adherence of FFPE sections to slide. -Prior to deparaffinization, heat slides overnight in a 60 degrees Celsius oven.-All steps in which Xylene is used should be performed in a fume hood.Western Blot Protocol...
Add the primary antibody diluted in PBS 1% BSA, 0.1% Tween-20, and 0.05% NaN3. Note:If you use a blocking peptide as a negative control, refer to ourPeptide Blocking Protocolfor WB. Incubate for 2–3 hours at room temperature or overnight at 4°C with gentle agitation. ...
一、Western Blot标准操作流程 样本制备→凝胶电泳→蛋白转膜→封闭→抗体孵育→检测显影 1、样本制备 1...