Western blot一抗稀释液 产品简介：Western blot一抗稀释液(Primary Antibody Dilution Buffer)可以用于免疫印迹中的一抗的稀释和配制，使用Leagene Western blot一抗稀释液配制的一抗，可以在4℃保存6个月，并且可以反复多次使用。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
碧云天生产的QuickBlock™ Western二抗稀释液(QuickBlock™ Secondary Antibody Dilution Buffer for Western Blot)是最新一代的高效二抗稀释液,总体效果显著优于传统的基于BSA(牛血清白蛋白)、脱脂奶粉等的二抗稀释液,可以用于Western blot (WB)时二抗的稀释和配制。 QuickBlock™ Western二抗稀释液可以有效节约二抗。
Western一抗稀释液 100ml/瓶 当日可发价格:价格电议产品详情:名称Western一抗稀释液规格100mL存储4℃保存,一年有效。产品简介Western一抗稀释液(Primary Antibody Dilution Buffer)可以用于... Western二抗稀释液 100ml/瓶 当日可发价格:价格电议产品详情:名称Western二抗稀释液规格100ml存储4℃保存,一年有效。产品简...
Figure 6: Serial Dilution of Secondary Antibody For optimal results, it is recommended to dilute the antibody according to the suggested concentration. Shorter incubation times for the secondary antibody can be employed, with a suggested incubation period of 1 hour at room temperature. Using blocking...
4112.00 商品介绍 商品咨询 在蛋白印迹(Western Blot , WB)实验中,一抗和二抗通常需要适当稀释使用。本产品可用于蛋白印迹时抗体的稀释和配制,稀释和配制的一抗或二抗可以在2-8℃保存和使用不少于1周,可以反复2-3次用于蛋白印迹,节约您宝贵的抗体。本产品仅用于WB实验中的抗体和标记物的稀释。0...
a.将一抗用Western Antibody Dilution Buffer按照合适比例进行稀释; b.封闭结束后,将稀释好的一抗工作液倒入孵育槽中,室温2h或4°C过夜。 c.一抗孵育结束后,加入TBSTBuffer洗涤4次,5min/次; 二抗孵育、洗涤: a.一抗洗涤结束前10min,将二抗用Western Antibody Dilution Buffer按照合适比例进行稀释; ...
NOTE: Please refer to primary antibody product webpage for recommended primary antibody dilution buffer and recommended antibody dilution. A. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit:#12957Western Blotting Ap...
The dilution should be optimized in preliminary experiments for the best results in terms of high signal and low background. Cut the appropriate area of the blot with a scalpel using the X-ray film as reference and wet by capillarity in the skimmed milk solution. Incubate with shaking for 1...
Conversely, adding too much blocking agent or detergent to the antibody dilution solution can prevent efficient binding of the antibody to the antigen, causing reduced signal as well as reduced background. Continue reading: Secondary An...
碧云天生产的Western溶液套装(封闭液、洗涤液、一抗和二抗稀释液),是一种包含了Western Blot实验过程中常用的封闭液、洗涤液、一抗和二抗稀释液的溶液套装。 本产品中的一抗稀释液(Primary Antibody Dilution Buffer for Western Blot) 可以用于Western时一抗(primary antibody)的稀释和配制。其主要有效成分为经过反复...