【解析】 Western blotting与 Southern blotting或 Northern blotting类似,但 Westen blotting采用的是聚丙烯酰胺疑肢电泳PAGE)被检测物是蛋白质,“探针”是抗体,“显色”用标记的二抗。经过PAGE分离的蛋白质样品,转移到固相载体如硝酸纤维素薄膜上,固相载体以非共价键形吸附蛋白质,且能保持电泳分离的多肽类型及其生物学...
免疫印迹法 (Western blotting) 是一种将高分辨率凝胶电泳和免疫化学分析技术相结合的杂交技术。通俗来讲,将蛋白质通过电转固相到膜上,然后利用抗体检测蛋白表达的方法。与Southern早先建立的检测核酸的印迹方法 Southern blot 相类似,亦被称...
Southern blotting, discovered in 1975 by E.M. Southern, represents a technique to detect a gene of interest in the DNA sample. The steps involved are isolation of DNA, its separation by electrophoresis, transfer to a suitable medium, hybridization to probes and visualization of the gene if it...
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百度试题 题目Southern Blotting. Northern Blotting和 Western Blotting分别是用于研究 和 的技术。相关知识点: 试题来源: 解析反馈 收藏
免疫印迹法(Western blotting) 是一种将高分辨率凝胶电泳和免疫化学分析技术相结合的杂交技术。通俗来讲,将蛋白质通过电转固相到膜上,然后利用抗体检测蛋白表达的方法。与Southern早先建立的检测核酸的印迹方法Southern blot相类似,亦被称为Western blot。 Western Blotting可用于: ...
描述进行WesternBlotting、NorthernBlotting和SouthernBlotting的实验目的和主要步骤。 (分数:2.00) ___相关知识点: 试题来源: 解析 正确答案:(正确答案:如表所示。) 解析:反馈 收藏
Southern, Northern and Western blotting Western blotting, also known as immunoblotting, is a technique used to detect specific proteins in protein samples. It involves the separation of proteins gel electrophoresis, followed the transfer of these proteins to a membrane and incubation with primary and...
Southern 印记杂交是指DNA与DNA的杂交。Northern 印记杂交是检测RNA的杂交。Western blotting 又称为蛋白质印记或免疫印记。 blotting为印记。前
百度试题 题目69. Southern Blotting、 Northern Blotting和 Western Blotting分别是用于研究 和 的技术相关知识点: 试题来源: 解析反馈 收藏