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The mixture of virus with immunoglobulin samples was then added to Vero E6 cells, and viral infectivity was assessed for the presence of infection using the Nano-Glo luciferase assay system (Promega, Madison, WI). Neutralizing activity was determined as a percentage after normalizing the data to ...
Spleens, brains, and IP lavage were collected from mice day six post-infection for analysis. Half of each organ (spleen and brain) was lysed using the Percellys lysis system and used for plaque forming unit (PFU) assay. Half of the spleens were processed for flow cytometer by disruption ...
Using site-directed mutagenesis of the WNV VLP system we identified amino acids (a.a.) important in hMAb binding. Residues V19 and L33 of the prM protein were determined to be the important sites for binding of all 3 hMAbs. Mutations at residues T20 and T24 had some affect on binding ...
whereas hemorrhagic flaviviruses such as DENV have more interactions between monomers and thus have less prominent holes.21The study postulates that the E dimer hole is functionally important for virus entry into the nervous system.21E-138 is one of nine electrostatic residues responsible for this un...
This wide distribution is because small changes near the start of the deployment can have drastic changes on the end trajectory due to cumulative changes over long periods of time in a complex system of currents. The conclusion of this analysis was that NOMAD lines needed some form of ...
The hydrography of the coastal waters of West Greenland is linked to the large-scale North Atlantic circulation system via the combined effects of the East Greenland Current (EGC) and the Irminger Current (IC), which merge to form the West Greenland Current (WGC; Figure 1). Thus, summer ...
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Deciphering the hydrothermal evolution of a VMS system by LA-ICP-MS using trace elements in pyrite: an example from the Bracemac-McLeod deposits, Abitibi, Canada, and implications for exploration Economic Geology, 110 (2015), pp. 2087-2108 CrossrefView in ScopusGoogle Scholar Gilbert et al.,...
The NS1 protein is essential for viral RNA replication, is involved in immune system evasion, and represents the major positive selection target during speciation of arthropod-born flaviviruses such as DENV and ZIKV [88]. NS2A and NS4B proteins have been shown to antagonize the interferon ...