transfer buffer (recipe here is for wet transfer) preparation is required for protein transfer. Sometimes, ponceau red staining is an alternative to check whether the protein transfer is successful, so a recipe of ponceau red staining solution is necessary. Besides, TBS buffer, blocking buffer, ...
Fig 1. Procedures of Western Blot wet transfer Buffers for wet Western Blot transfer 1Xtransfer buffer (25 mM Tris, 192 mM glycine, 20% methanol) recipe For 1.0 L:3.0 g Tris-base 14.4 g glycine 200 mL methanol Add ddH2O to 1 L. ...
Transfer buffer (wet, makes 1000ml) Tris-base 2.43 g Glycine 11.26 g Methanol 200 ml Fully dissolve the compounds and mix. Add water to make up to 1000 ml. Store in dark. ** If the weight of target protein is bigger than 100 kD, we recommend adding 0.04% SDS in transfer buff...