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For the biofilm samples, coupons made of the same material as the reservoirs (concrete or coated steel) were placed at two different depths to evaluate the effects of water level fluctuations. All samples were monitored for nitrifying bacteria, coliforms and E. coli by polymerase chain reaction ...
Fig. 2: Legionella counts during the first 8 weeks of biofilm development on EPDM coupons in non-chlorinated tap water at 37 °C. a Culturable L. pneumophila measured with Legiolert (n = 6). b Legionella spp. and L. pneumophila gene copies (gc) measured with ddPCR (n = ...
In general, higher HPC and AOB levels were measured on the biofilm coupons placed on the bottom of the reservoirs (area constantly submerged) than on top of the reservoirs (intermittently exposed to air). In the liquid samples, the upstream reservoir exhibited higher HPC and AOB levels than ...