14 of 21 dagen, afhankelijk van de bestemmingen waarop was gevlogen en de actuele regelgeving. Na afloop van de quarantaine stond de bemanning gedurende nog eens 7-14 dagen thuis onder medisch toezicht met dagelijkse temperatuurcontroles en PCR-vervolgtests. ...
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Quantitative PCR was performed in 10 μL reaction volumes using the NovoStart® SYBR qPCR SuperMix Plus kit (Novoprotein, China) on the Stepone Real-Time PCR System (Applied Biosystems, USA). In this specific analysis, the relative expression level of 16S ribosomal RNA was normalized to the...
(GCN2 KO) or act as control, respectively, and then maintained these mice on a control or leucine-deficient diet for 3 days. RT-PCR and western blot analyses revealed that GCN2 levels were decreased by more than 50% in the amygdala, but not in other brain areas [such as the arcuate ...
aThe DASL-based discovery method that we describe here should be distinguished from candidate-gene profiling methods based on the reverse transcriptase (RT)-PCR assay, such as those used in the commercially available OncotypeDx test for determining the prognosis in patients with breast cancer.18 Wher...
∗p < 0.05, control diet WT versus control diet KO; #p < 0.05, control diet WT versus ω3-rich diet WT; and $p < 0.05, control diet WT versus ω3-rich diet KO by ANOVA Dunnett’s test. (H) Quantitative RT-PCR of Ucp1 in sWAT of WT and KO mice fed a control or ω3 ...
We previously reported brown adipocytes can proliferate even after differentiation. To test the involvement of mature adipocyte proliferation in cell number control in fat tissue, we generated transgenic (Tg) mice over-expressing cell-cycle inhibitory pr
The absence of mycoplasma was verified by PCR analysis (PCR Mycoplasma Test Kit I/C, PromoKine, PromoCell France). 4.4. RNA Isolation, RT-PCR, qPCR Total RNA was isolated from differentiated adipocytes (LiSa-2 and SGBS) using Trizol (Invitrogen Life Technologies, Waltham, MA, USA), ...
Relative humidity is the percentage of water vapor present in the air, and it can vary as the air temperature changes [23]. 3.1.2. Light Intensity One of the factors that significantly influences plant growth is light intensity [24]. Plants are exposed to varying levels of light intensity ...
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