The CDS of VvCPR1 was amplified using the Prime STAR™ HS DNA polymerase (TaKaRa, Dalian, China) with the specific primer pairs (Forward: 5′-ATGCAATCGAGCTCTGTGAAG-3′; Reverse: 5′-TCTAGAGATCCCTGCTCTTGC-3′) as previously described23. PCR products were then cloned into pMD 18-T (...