ADA/Luc or HXB2/Luc were generated by cotransfection of luciferase reporter gene vector, pNL4-3-Luc-E- R-, and one of two envelope expressing vectors, pSMADA (R5) or pSMHXB2 (X4). The VSV-G/Luc or VSV-G/GFP were
Transplantation with a lineage-depleted, gene-modified cell product induced antigen-specific CD4+ and CD8+ T cells against the VSV-G envelope. These T cells had the ability to suppress a VSV-G-expressing cell line following transplant as compared to a parental cell line lacking expression, ...
For MRC-5 transductions and genetic reprogramming, retroviral vectors were also prepared with the tritransfection method where the RF-encoding vector, a GAG POL-expressing plasmid and the ecotropic envelope were co-transfected in HEK-293T cells (ratio 40, 40, and 20%, respectively). Procedures ...
Therefore the 3′ LTR U3 region of the gene transfer vector plasmid can serve as the 5′ LTR U3 promoter involved in gene expression in the genome of target cells (FIG. 3). Thus, a vector was prepared in which the 3′ LTR U3 region of the SIVagm gene transfer vector was replaced ...
terminal of VSV-G is permissive for short peptide insertion [20]. We next assessed the incorporation of the chimaeras into lentiviral particles. To this aim, expression vectors for VSV-G, αMHC/VSV-G and αHEL/VSV-G were co-transfected with the pCMV△R8.2 helper plasmid expressing Gag...
envelope proteins to alter host tropism, which may result in enhanced infection of the recombinant virus. The G glycoprotein of the vesicular stomatitis virus glycoprotein (VSVG) is used in this invention towards engineering exosomes with specific membrane-bound proteins by expressing gene-encoding V...
ADA/Luc or HXB2/Luc were generated by cotransfection of luciferase reporter gene vector, pNL4-3-Lue-E–R–, and one of two envelope expressing vectors, pSMADA (R5) or pSMHXB2 (X4). The VSV-G/Luc or VSV-G/GFP were produced by a three-plasmid expression system which consisted of ...