肠球菌感染的增加主要由于耐药基因的获得。对万古霉素的耐药性通常由九种van基因(vanA、vanB、vanC、vanD、vanE、vanG、vanL、vanM和vanN.1-5)中的一种介导。其中,vanA和vanB是最常见的基因型。VanAVRE 对万古霉素和替考拉宁均有耐药性,而VanBVRE 对万古霉素耐药...
Consecutive Enterococcus faecium isolates phenotypically suspected and subsequently confirmed to be vanB positive by PCR over 18 months were analysed using the Bruker microflex. Control groups were similarly analysed and the ability of MALDI-TOF MS to identify VRE was calculated using statistical tools ...
Experience with the Roche LightCycler VRE detection kit during a large outbreak of vanB2/B3 vancomycin-resistant Enterococcus faecium. Comment on J Antimicrob Chemother. 2007 Apr;59(4):809-10. TH Koh,RN Deepak,SY Se-Thoe,... - 《Journal of Antimicrobial Chemotherapy》 被引量: 17发表: 2007...
(2)毒力基因扩增:使用5重PCR的方法扩增艰难梭菌的16S rDNA、毒素A、B编码基因tcdA、tcdB,以及二元毒素基因cdtA、cdtB,并对tcdA基因是否存在1.8kb片段缺失进行确证,扩增产物使用1.5%的琼脂糖凝胶电泳判定结果。 04 产毒素培养(Toxigenic culture, TC):粪便培养分离艰难梭菌,再使用PCR方法检测菌株是否含有毒素基因。
vanB PCR vanB We present here occurence of PCR-positive but culture-negative for vanB vancomycin-resistant enterococci (VRE) from an enrichment broth of a stool surveill... 원다해,홍기호,윤경아,... - 《Laboratory Medicine Online》 被引量: 0发表: 2013年 Abstracts to be ...
For instance, Van der Elst and Bosch [80] observed that limited budgets under the Dutch SDE + scheme have led to under-bidding for support application. Many investment decisions will be finally rejected, once the project viability becomes clear [80]. A similar case is also reported for PV ...
(Fig.1B). The presence ofvanAandvanBwas investigated in all isolates by PCR assays. Consistently, we confirmed the presence of the entirevanAcluster in 54 out of the 55 sequenced genomes. Of note, the genome of ERV69 lacked the two-component regulatory systemvanSR, although it still ...
VanB, enterococcus faecium and cc17 PCRs were performed on environmental surfaces after disinfection. By this combined PCR, both vancomycin-susceptible (VSE) as well as the cc17+ VRE outbreak-strain were identified. Beds, bed bells, bedside tables and door knobs were considered high-touch surfac...
5~ 4 2 8 2~ 64 64 64 2~ 64 10 10 10~ 10 10 10 10~ 10 , AUC [ 1617] v anA vanB , M IC 2 ~ ( Aminoethoxyacetic acid, A) 90 4g/ mL ( B) ( 30% , 10% A, 40% B) ( 3% A, 6% ( 3log ) B) P450 - , , ( AB ) , , , , Cmax AUC 600mg/ 12h ( ) 15...
The iNtRON vancomycin-resistant enterococci (VRE) vanA/vanB real-time PCR assay was directly applied to stool surveillance (direct-PCR). direct-PCR was compared to 2 culture-based methods using Enterococcosel broth (enrichment-culture) and ChromID VRE media. The positive broth of the enrichment...