Gene C16ORF62Applications/DilutionsDilutions Western Blot Application Notes This product is intended for use as a positive control in Western Blot.Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over...
Mouse liver total RNA was isolated by ISOGEN (Nippon Gene, Japan) and first-strand cDNA generated using the Invitrogen SuperScriptIV First-Strand Synthesis system with random hexamers. For quantitative real-time PCR, the StepOnePlus real-time PCR system (Thermo Fisher) was used, and the PCR ...