Surprisingly, fusion of MEIS1 to the Vp16 transactivation domain (but not the engrailed transrepression domain) produced an autonomous oncoprotein that immortalized progenitors and caused myeloid leukemias without the need for coexpressed exogenous or endogenous Hox genes. Like MEIS1, Vp16MEIS1 ...
在α 疱疹病毒中 UL48编码的 VP16同源蛋白都含有转录激活 域(Transactivationdomain,TAD )和核心区( VPl6-inducedcomplex,VIC)。转录激活域富含酸性氨基酸, HSV的VP16TAD 位于第412至490位氨基酸残基,可分为两个子区域并且各自能够独立或协同执行激活转录功能,子区域分别被称为氨基域 (VP16N或VP16412-456)、羧基...
(serine is replaced by alanine) that prevents both phosphorylation at this site andtransactivationof genes by CREB[14,17,18]. This mutation does not affect the ability of mCREB to dimerize with endogenous (wild-type) CREB or another mCREB molecule, nor does it affect binding at CRE sites ...
Many transcription factors can activate the initiation of DNA replication. We have used affinity chromatography to show that the acidic activation domains of the transcription factors VP16, GAL4, and p53 each bind selectively to human and yeast replication factor A (RPA). The binding is direct an...
For changes at the critical phenylalanine residue at position 442 of VP16 there was a good correlation between transactivation activity in vivo and the binding of VP16 to TFIID in vitro. In contrast, mutants with reduced negative charge were more defective for binding than for activation....
HVT UL48 protein lacks the acidic C-terminal tail, known to possess the transactivation capacity of HSV-1 VP16. Hydrophobic cluster analysis has revealed that its predicted domain composition is closely related to ORF 10 transactivator protein of VZV.Kopacek...
which may contribute to the amelioration of the skin lesions in an HSV-1 infection mediated zosteriform model.Our study provides new insights into the mechanisms by which Hsp90α facilitates the transactivation of HSV-1 α genes and viral infection, and highlights the importance of developing sele...
Coexpression analysis revealed that VP16 selectively blocked IFN regulatory factor 3 (IRF-3)-mediated but not IRF-7-mediated transactivation. VP16 was able to bind to IRF-3 but not IRF-7 in vivo, based on coimmunoprecipitation analysis, but it did not affect IRF-3 dimerization, nuclear ...
Because the molecular interactions that underlie the activation of transcription by VP16-CREB and phospho-CREB are different, the transactivation driven by the VP16 domain might have a broader range than that of CREB. It is therefore possible that the binding of VP16-CREB to the CRE element ...
We sought to exploit this native adaptive response to hypoxia as a treatment for chronic ischemia.Methods and Results—A hybrid protein consisting of DNA-binding and dimerization domains from the HIF-1α subunit and the transactivation domain from herpes simplex virus VP16 protein was constructed to...