VP16 interacts via its activation domain with VP22, a tegument protein of herpes simplex virus, and is relocated to a novel macromolecular assembly in coexpressing cells - Elliott, Mouzakitis, et al. - 1995Elliott, G., Mouzakitis, G. & O'Hare, P. (1995). VP16 interac...
转录因子包含DNA binding domain (BD)和activation domain (AD),而且不同转录因子的BD和AD是可以互换而不影响转录激活的.你想证明是activation domain,就融合到一个已知转录因子的BD里,常用的就比如Gal4,LexA的BD,然后去检测reporter gene的转录情况.如果有转录发生,则转录因子完整,如果没有检测到转录,则转录因子不...
Comparison with published activator structures reveals that the VP16 activation domain uses distinct interaction modes to adapt to unrelated target surfaces and folds that evolved for activator binding. 全文 全文 访问全文 收藏 分享 被引(102) 浏览 更新时间:2024-04-15 11:48 相似论文 引用论文 ...
Pdx-1 fusion to VP16 activation domain did not further enhance insulin promoter activity. Figure 2 Synthesis and biological activity of Pdx-1, TAT-Pdx-1, Pdx-1-VP16 and TAT-Pdx-1-VP16 fusion proteins. (a) Schematic structure (top panel) and purity (bottom panel) of Pdx-1, TAT-Pdx-...
Structural features of the transcriptional activation domain of the herpes simplex virion protein VP16 were examined by oligonucleotide-directed mutagenesi... JL Regier,F Shen,SJ Triezenberg - 《Proc Natl Acad Sci U S A》 被引量: 463发表: 1993年 Fusion of GAL4-VP16 to a steroid-binding ...
Major insights into the regulation of chromatin organization have stemmed from biochemical studies using Gal4-VP16, a chimeric transcriptional activator in which the DNA binding domain of Gal4p is fused to the activation domain of viral protein VP16. Unexpectedly, given previous intensive efforts to...
Because the molecular interactions that underlie the activation of transcription by VP16-CREB and phospho-CREB are different, the transactivation driven by the VP16 domain might have a broader range than that of CREB. It is therefore possible that the binding of VP16-CREB to the CRE element ...
A synergistic increase in potency of a multimerised VP16 transcriptional activation domain. EMBO J. 1992; 11 :5005–5012.Emami, K. H., and M. Carey. 1992. A synergistic increase in potency of a multimerized VP16 transcriptional activation domain. EMBO J. 11 : 5005-5012....
Many transcription factors can activate the initiation of DNA replication. We have used affinity chromatography to show that the acidic activation domains of the transcription factors VP16, GAL4, and p53 each bind selectively to human and yeast replicati
The Novel Transcription Factor e(y)2 Interacts with TAFII40 and Potentiates Transcription Activation on Chromatin Templates Finally, using an in vitro transcription system, we demonstrate that recombinant e(y)2 is able to enhance transactivation by GAL4-VP16 on chromatin but... S Georgieva,E Nab...