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We used these v1 ZF-DdCBEs as the starting point for development and optimization. We hypothesized that ZF-DdCBE editing outcomes might be limited if the linker between the ZF array and the split DddA deaminase constrained access of reassembled DddA to the target nucleotide(s). We replaced ...
Another point to mention is the dose used for this study. In the in vitro assay, NVP-BGT226 showed CC50 value of 2.66 μM, but no sign of toxicity was observed in vivo at 5 mg/kg dose. Since the dose was selected based on the previously reported literature that does not show ...
To characterize this, the signal of the detector was measured at different radial and transversal distances from the effective point of measurement of the PSD to the center of the Ir-192 source using a PMMA phantom submerged in a water scanning phantom. The PMMA phantom has two identical ...
Test strategy for assessing the risks of nanomaterials in the environment considering general regulatory procedures. Environ. Sci. Eur. 2015, 27, 1–12. [Google Scholar] [CrossRef] [PubMed] Stone, V.; Johnston, H.; Schins, R.P.F. Development of in vitro systems for nanotoxicology: ...
The assay has been used successfully on a rather small number of biological samples and can provide only end-point data. FAS enzyme activity has been measured on most different sample types by radioassay; however, this requires special equipment and the ability to work with radioactive material,...