h, Dorsal view (anterior to top) of embryos hybridized with a probe against snail2; black arrows point to migrating neural crest. i, Percentage of embryos displaying neural crest migration. Histograms show mean, error bars represent s.e.m.; n = 13 embryos; two-tailed t-test, **P...
Fig. 3: Single-cell mRNA sequencing of cultured cells reveals a large number (11) of cellular populations. aUMAP plot of 9561 cells, taken from cultures produced from an embryo’s entire hemisphere, classified based on the top ten most differentially expressed genes (DEGs), as shown in the ...
It was observed that the cells began to proliferate at day 6, which is the key time point when PBMCs differentiate into fibrocytes. The cells must be passaged according to their proliferative status, and purified fibrocytes could be obtained at day 12 (75%). The purified fibrocytes were ...
Some endocrine therapies, such as tamoxifen, have been reported to display weak agonistic activity in the endometrium due to altered cofactor recruitment and subsequent activation of gene transcription40. We therefore sought to test whether AZ’6421 was a complete antagonist or could act as a partial...
Activation of free fatty acid receptor 2 (FFAR2) by microbiota-derived metabolites (e.g., propionate) reduces leukaemic cell proliferation in vitro. This study aims to test whether Ffar2 expression per se also influences leukaemia cell growth in vivo. Bcr-Abl-expressing BaF cells were used as...
To test whether the presence of specks invariably is associated with cell death, we stained activated cells with 7-amino-actinomycin D (7AAD), a vital dye with a high affinity for DNA that does not pass through the intact cell membrane. As seen in the histograms plotted in Figures 2A and...
To identify significant protein alterations between the mdx and wild-type groups, the mean relative ratio (unlabeled/labeled values) was compared for each protein between mdx (n = 3) and control (n = 3) mice using a non-parametric Wilcoxon rank sum test. Significance was set at p < 0.05...
classifier, we trained on one-channel EAP features (TPW, REP) of in vivo units to discriminate between FS and RS clusters (training/validation set, 75%/25%), and classification accuracy on the validation data set exceeded 99% (SVM; Fig.4g). When applying the classifier on the test ...
Recent advances in base editing have created an exciting opportunity to precisely correct disease-causing mutations. However, the large size of base editors and their inherited off-target activities pose challenges for in vivo base editing. Moreover, the
5a). Among the cancer types, pancreatic ductal carcinoma (PDAC; p = 0.049; t-test) and colorectal carcinoma (CRC; p = 0.0035; t-test) were comparably reconstructed between PDX and cell lines, as compared to skin cutaneous melanoma (SKCM; p = < 0.0001; t-test), breast...