After 4 hours, the solvent was removed under vacuum and the crude product was purified in a silica flash column using diethyl ether as eluent. WOS-Cy7 was obtained as red-brown oil (61 mg, 0.06 mmol, 36 % yield). 1H NMR (400 MHz, CD3OD) δ = 7.15 (s, 1H), ...
To efficiently conductin vivosingle-cell experiments, the first task for ACE is to maximize the successful detection of neurons. In previous whole-cell studies automatic neuron detection was achieved by passively monitoring the change in pipette resistance (Rp) as the pipette advanced into the brain...
The BUCHI pure chromatography system (BUCHI, Pure C-850 FlashPrep, Flawil, Switzerland) was used for DK separation. Chromatography was performed on Agilent Technologies 1220 Infinity II LC with a column (poroshell 120 C18, 4.6*100 mm, 4µm). The mobile phase consisted of A; DW (+0.1% ...