The PCR products were treated with FD DpnI at 37 °C in a heat block for 5 min to remove the intact template plasmids before being transformed into DH5α competent E. coli. The resulting mutated plasmids were further transformed into the engineered strain. The primers were designed ...
International Journal of Molecular Sciences Article Vitamin C Protects Chondrocytes against Monosodium Iodoacetate-Induced Osteoarthritis by Multiple Pathways Pu-Rong Chiu 1,2, Yu-Chen Hu 2, Tzu-Ching Huang 1,2, Bau-Shan Hsieh 2, Jou-Pei Yeh 2, Hsiao-Ling Cheng 3, Li-Wen Huang 4,*,† ...
Afterward, 300 µL of MTT solution (5 mg/mL) was added to each well in the growth medium for 3 h at 37 ◦C in the dark. The MTT solution was removed, and the formazan crystals were dissolved in 1 mL of DMSO/well for 20 min. The optical density of solubilized formazan in ...
Scheme 1. Chemical structure of poly(3,4-ethylenedioxythiohene) (PEDOT; left) and pSSocclhhyee(mhmyeed1r.o1Cx.yhmemCetihcheaymlls-it3cr,ua4lc-etuthrseytrlouefncpteuodrliyeo(x3,y4ot-hfetihoypplhoeenlyne(de3)i,o4(xP-eyHtthhMyioleheEneDnedeO)iTo(Px; EyriDtghOhiotT)h;.elenfet)) ...
Blan2k NPs were prepared 0as described above 20 but without the2a5d%dPit1i2o3n/7o5f%RT19P.GNS R-labeled N2Ps were prepared by 5replacing R19 with NR (0.2 mg per5N0%PPf1o2r3m/5u0l%atiToPnG),Sand the NPs2 were purified by di1a0lysis, as described above. A schema7t5i%c oP1f10...