6Sample Collection and Storage...7Detailed Protocol... 8-9Calculations... 10Measurement Range... 10Troubleshooting... 10Reagent Stability...
Transfection was performed according to the manufacturer’s protocol (Invitrogen Corporation). Briefly, the cells were seeded in wells of a 6-well plate at 1 × 105 cells/well and incubated in RPMI 1640 medium. When the cells had reached confluence, OPTI-MEM I Reduced Serum Medium was ...