5Assembly of Viral Matrix Proteins Thematrix proteinsof negative-strandRNA virusesare initially synthesized assoluble proteinsin the cytoplasm, which then partition into membranes to varying extents, depending on the type of virus and experimental conditions. For most viruses, association with membrane...
Viral-like particles (VLPs) are nanoscale structures made up of assembled viral proteins that lack viral genetic material and are therefore non-infectious [1]. VLPs are dispersed nanomaterials that can be produced in a variety of systems, including mammals, plants, insects, and bacteria. VLPs can...
Respiratory syncytial virus (RSV) is a leading cause of lower respiratory tract disease in young children and elderly people. Although the virus was isolated in 1955, an effective RSV vaccine has not been developed, and the only licensed intervention is passive immunoprophylaxis of high-risk infant...
In mature virions, the structural proteins are inserted into the host-derived lipid bilayer in an icosahedral architecture where 180 units of the E protein cover the surface of the virion in 90 head-to-tail homodimers. The E protein is the main target of the neutralizing antibody response ...
Purified, inactivated whole virus vaccines have been developed to circumvent issues associated with live-attenuated vaccines. This approach eliminates the possibility of viral replication yet retains, to varying degrees, theantigenicityof the structural proteins. Inactivated viral vaccines are considered desira...
Autophagy is defined as a general term for the degradation of 'old' lysosomal proteins and damaged organelles[13], which takes on a pro-survival role by supplying cells with the nutrients required to support metabolism when cells are under stress[14]. However, it has been identified that autop...
only the longest transcript assembled for each gene. BUSCO v5.2.1 [41] was then used to assess transcriptome completeness using the endopterygota_odb10 lineage. The scripts used for this pipeline are available athttps://github.com/sarahinwood/asw-transcriptome. The transcriptome assembly was ...
"There have been unfriendly folks trying to misinterpret our work as gain-of-function research. Let me be clear – that is not the case. What we've done is simply tested a passaged virus mutant, nothing more. The ACE2 humanized mice used in our experiments are unique and do not exist...
of ZIKV proteins in captured sterol–protein complexes by western blot using antibodies specified on the right. Molecular weights are indicated on the left (in kilodalton; kDa). A representative result is shown (n = 4). Samples without UV irradiation but otherwise processed as inbwere used...
All of these proteins are present in mature, infectious virions, in which the negative-strand, genomic RNA is coated with the N protein (one N per 9 ribonucleotides) to form a ribonucleoprotein (RNP) complex3. The VSV RNP complex assembles, together with M, into a helical, bullet-...