Full size image pT181-Qß vaccination reduces both soluble and insoluble species of pTau Accumulation of hyperphosphorylated tau in the hippocampus and the cortex is a hallmark of tauopathies. In order to determine the efficacy of pT181-Qß vaccination, we immunized rTg4510 mice with either ...
123 Movies were recorded for a total exposure of 4.5 s with 40 frames per movie and a total dose of 40 e−/Å2. The magnification was 36,000x (1.15 Å/pixel at the camera level). The defocus of the images was changed between −1.0 and −2.5 μm. Two different datasets ...
Using SerialEM (v3.7.1–64 bit)41, we recorded a total of 27 movies for each stage position (nanoprobe mode with an illuminated area of ~1 μm, 3 exposures per hole from a total of 9 holes). Cryo-EM data processing Particle picking and movie processing Movie frames were aligned ...
Viral assembly occurs at the plasma membrane. For an influenza particle to be fully infectious, the eight vRNPs must be packaged in a virion. Virions do not usually package more than eight segments7and each segment generally occurs once per virion. In agreement, full-length segments compete wi...
WT brains was predominantly localized in cerebral cortex, with weak signals in the olfactory bulb and with no detectable infection in lateral ventricles nor the fourth ventricle (Fig.1cinset ii, Supplementary Fig.1c, and Supplementary Movie1). In contrast, inIfnar–/–brains, we observed weak ...
Full size image This approach yielded 21 distinct structures at resolutions of 6–9.1 Å of RSV hexamer/pentamer, hexamer/hexamer, and pentamer/pentamer pairs that exist in mature RSV CA lattice (Fig.3b, Supplementary Fig.3b, and Supplementary Movie3) and also yielded a structure of the...