The following antibodies were used: mouse monoclonal anti-pan-vinculin (which can detect both vinculin and metavinculin forms) (clone hVIN-1, Sigma, V9131); mouse monoclonal anti-sarcomeric-α-actinin (clone EA-53, Sigma, A7811); rabbit polyclonal anti-pan-cadherin (Sigma, C3678); rabbit ...
Mouse anti-vinculin (Sigma #V9131) at 1:400. Secondary antibodies Alexa Fluor (Abcam) against the specific species were diluted 1:400 in PHEM buffer 3% BSA. Samples were hard-mounted using DAKO mounting media. Confocal imaging of immunostained samples was performed using a spinning-disc ...
Sigma-Aldrich V9131 Monoclonal Anti-Vinculin antibody produced in mouse Quick View Sigma-Aldrich V4505 Monoclonal Anti-Vinculin antibody produced in mouse Quick View Sigma-Aldrich V9264 Anti-Vinculin antibody, Mouse monoclonal Quick View Sigma-Aldrich SAB4503069 Anti-Vinculin antibody produced in rabbit...
Mouse anti-vinculin antibody (clone hVin1) (Sigma, UK) was diluted (1:500) in 1% Bovine Serum Albumin (BSA) (cat: V9131, Sigma, UK). Dylight 594-conjugated AffiniPure Donkey Anti-Mouse IgG (cat: 715-585-150, Jackson ImmunoResearch, USA) was used as a secondary antibody, diluted in...
Mouse anti-vinculin antibody (clone hVin1) (Sigma, UK) was diluted (1:500) in 1% Bovine Serum Albumin (BSA) (cat: V9131, Sigma, UK). Dylight 594-conjugated AffiniPure Donkey Anti-Mouse IgG (cat: 715-585-150, Jackson ImmunoResearch, USA) was used as a secondary antibody, diluted in...
Force transmission through adherens junctions (AJs) is crucial for multicellular organization, wound healing and tissue regeneration. Recent studies shed light on the molecular mechanisms of mechanotransduction at the AJs. However, the canonical model fa