The apparatus included a flow-guiding, non-conducting template which was equipped with one or more liquid inlets, liquid outlets, and efflux channels inside the template. The apparatus included a source of direct current, the negative pole of which was connected by way of a pulse generator ...
FIG. 2 shows the expression profiling of LpCAD1L genes using LpCAD1 as template gene in perennial ryegrass as log ratios of genes matching LpCAD1 at an Euclidian distance of 4.4393. FIG. 3 shows the nucleotide sequence of the cDNA fragment LpCAD1-La (Seq ID No. 1). FIG. 4 shows pl...
Following prestimulation, 2.0×106 cells were seeded per well of a 24 well plate in 400 μL of bone marrow culture medium, plus 4 μg/mL polybrene (Sigma) and 10 mM HEPES (Gibco-Invitrogen). 75-150 μL of retrovirus was added to the cells to give an MOI of what our method of ti...
Alternatively, the gene sequence encoding the GRF/PACAP precursor polypeptide (i.e. the genomic sequence including introns) or pieces thereof may be obtained by amplification using primers based on the presented gene sequence and genomic chicken DNA as a template. V. Production of GRF/PACAP sequen...
number of nucleotides in each strand which allows them to reduce the level of NR2F2 protein, or the level of a nucleic acid encoding NR2F2. The dsRNA molecules provided herein can be chemically synthesized, or can be transcribed in vitro from a DNA template, or in vivo from, e.g., ...