FIG. 2 shows the expression profiling of LpCAD1L genes using LpCAD1 as template gene in perennial ryegrass as log ratios of genes matching LpCAD1 at an Euclidian distance of 4.4393. FIG. 3 shows the nucleotide sequence of the cDNA fragment LpCAD1-La (Seq ID No. 1). FIG. 4 shows pl...
The apparatus included a flow-guiding, non-conducting template which was equipped with one or more liquid inlets, liquid outlets, and efflux channels inside the template. The apparatus included a source of direct current, the negative pole of which was connected by way of a pulse generator ...
Alternatively, the gene sequence encoding the GRF/PACAP precursor polypeptide (i.e. the genomic sequence including introns) or pieces thereof may be obtained by amplification using primers based on the presented gene sequence and genomic chicken DNA as a template. V. Production of GRF/PACAP sequen...
Following prestimulation, 2.0×106 cells were seeded per well of a 24 well plate in 400 μL of bone marrow culture medium, plus 4 μg/mL polybrene (Sigma) and 10 mM HEPES (Gibco-Invitrogen). 75-150 μL of retrovirus was added to the cells to give an MOI of what our method of ti...
number of nucleotides in each strand which allows them to reduce the level of NR2F2 protein, or the level of a nucleic acid encoding NR2F2. The dsRNA molecules provided herein can be chemically synthesized, or can be transcribed in vitro from a DNA template, or in vivo from, e.g., ...