The hypotonic treatment in itself did not significantly affect the cell viability; however, electric field parameters for electroporation should be carefully selected taking into account the hypotonically induced volume increase of cells. 展开 关键词: hypotonic treatment cell swelling regulatory volume ...
The viability of cell lots after isolation is typically assessed via trypan blue staining, in which dead cells stain blue[10, 44, 45]. The viability of cells can be confirmed using two different dyes: trypan blue exclusion and [3H]leucine uptake[22]. To assess viability, Annexin V, to id...
Flow cytometric enumeration and immunophenotyping of hematopoietic stem and progenitor cells This approach allows the absolute CD34+ cell count to be determined directly from a flow cytometer. The method can be extended with a viability stain ... SM Keeney - 《Seminars in Hematology》 被引量: 179...
Excerpt This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates. This chapter describes assays where data are recorded using a plate-reader; it does not... TL Riss,AL Niles,L Minor - PubMed 被引量: 33发...
7.细胞活力(cell viability):细胞活力(cell viability of control)=(药物组A值-调零孔A值)/(对照孔A值-调零孔A值)*100%查 … www.antpedia.com|基于27个网页 3. 细胞活性 ... 细胞染色( Cellular Staning)细胞活性(Cell Viability) 活性氧检测( Reactive Oxygen Detection) ... ...
Artificial Cells Blood Substitutes & Immobilization BiotechnologyHan DW,Park HK,Park YH,et al.Beneficial effects of freezing rate determined by indirect thermophysical calculation on cell via-bility in cryopreserved tissues. Artif Cells Blood Substit Biotech-nol . 2006...
This effect, however, remains less well known than the toxic effect of ultraviolet light on living cells. Early embryonic development is characterised by rapid cell division and embryonic gene activation, which make embryos extremely vulnerable and sensitive to environmental influences. The human body ...
These studies demonstrate that the CellTiter-Glo(R) Assay can be used to assess primary cell culture viability, including that ofprimordial/stem cells. The CellTiter-Glo(R) Assay provides ahomogeneous assay, sensitive to four cells, allowing researchers to obtain reliable viability data while conserv...
The obtained results indicated that UV filters adversely affected the viability of nerve cells, most likely by enhancing the process of apoptosis. The most potent effect was exerted by BP-3 and 4-MBC and at concentrations that may be reachedin vivo. Since human exposure to UV filters is ...
cells is determined by the software. For every sample you run, the instrument requires 500 µL of sample volume, which is mixed 1:1 with trypan blue, and takes 50 images to determine the cell concentration and viability. All of this data is output on the computer screen and can ...