The Vi-CELL BLU cell counter and cell viability analyzer automates the Trypan Blue Dye Exclusion method for reliable and efficient cell analysis.
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Aspiration and Trypan Blue Mixing Out of Range Concentration Flag Filled Dispense Tray Flag Bioprocess 3D, Rotateable Plotting Export Multi-run Files to MS Excel Upgraded Audit Trail Supports 21 CFR Part 11 Non-viable Cell Declustering User-defineable Declustering Options Added Preferences for Secured...
The Vi-Cell system default cell type may work for some cell lines in the lab and it is really the best place to start. However, since trypan blue uptake, cell size, and appearance are unique to each cell type you should run some tests to determine the best parameters with your ...
The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method 鈥 B眉rker chamber and trypan blue dye for dead cell...
see pg. 49 in the user manual. Running a Sample All reagents including the trypan blue are internal to the instrument so all you need is your cell solution to get started. Sample cups are located on the shelf above the instrument.
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Cells were lysed by sonication and cell lysate was clarified by centrifugation at 17,000g, 4 °C for 0.5 h. The supernatant was bound to 5 ml Nickel-NTA affinity resin for 1 h at 4 °C. Supernatant was discarded and resin was washed 5 × 30 ml wash buffer (20...
Vi-CELL® XR Cell Viability Analyzer The Vi-CELL® XR Cell Viability Analyzer is a benchtop video imaging system that also uses the trypan blue technique for determination of cell viability. An external PC is required to queue samples, run the complex uidics system, and analyze ...
Cells were lysed by sonication and cell lysate was clarified by centrifugation at 17,000g, 4 °C for 0.5 h. The supernatant was bound to 5 ml Nickel-NTA affinity resin for 1 h at 4 °C. Supernatant was discarded and resin was washed 5 × 30 ml wash buffer (20...