The Vi-Cell system default cell type may work for some cell lines in the lab and it is really the best place to start. However, since trypan blue uptake, cell size, and appearance are unique to each cell type you should run some tests to determine the best parameters with your ...
Many cell culture facilities, expanding cells in bioreactors or flask cultures, use the standard, manual, trypan blue vital dye-exclusion cell-viability assay. Viable celts, given their intact plasma membranes, exclude the trypan bine stain; nonviable, membrane-permeable cells stain dark blue. This...
The correct cell count and adequate viability of DC are one of the quality control criteria for the final product release. Number of viable DC is historically determined by microscopy using manual counting method 鈥 B眉rker chamber and trypan blue dye for dead cell...
Cells were lysed by sonication and cell lysate was clarified by centrifugation at 17,000g, 4 °C for 0.5 h. The supernatant was bound to 5 ml Nickel-NTA affinity resin for 1 h at 4 °C. Supernatant was discarded and resin was washed 5 × 30 ml wash buffer (20...
Vi-CellXRCellViabilityAnalyzer UserInstructions WrittenbyCMCresson15-Feb-06 TheVi-CellXRCellViabilityAnalyzerisavideoimagingsystemusedtoanalyzeyeast,insect andmammaliancells.Itautomatesthetrypanblueexclusionprotocol,inwhichdeadcellstake upthedyewhilelivecellsdonot,andprovidesdataonpercentviabilityandcellcounts. Ess...
a, Expression of Ocr from the pBAD vector in PARIS+ culture resulted in cell toxicity, whereas the empty vector (EV) had no impact. b, Ocr expression in PARIS+ cells induced cell death (propidium iodide staining, red) and DNA compactization (DAPI staining, blue). Arrows indicate zoomed-...
In sum, our informatic analysis reveals that DUF4123 genes co-occur with five distinct families of unrelated T6SS effectors and that each of these effectors likely requires a cognate DUF4123 protein for its T6SS-dependent export from the cell. Fig. 1: Divergent sub-families of DUF4123 ...
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The type VI secretion system (T6SS) of Gram-negative bacteria inhibits competitor cells through contact-dependent translocation of toxic effector proteins. In Proteobacteria, the T6SS is anchored to the cell envelope through a megadalton-sized membrane c
BiFC signal is in blue, plastid autofluorescence is in red. Location of the cell nucleus is indicated by a white arrowhead. Images are single confocal sections. Bars = 30 μ m. functions in the plant cell, either in the cytoplasm or in the nucleus. For example, it is ...