Here, we have selected a representative VHH and tested two humanization hypotheses to accurately assess the potential impact of these changes. This example shows that despite the non-negligible change (1/10th of residues) brought about by humanization, the effect is not drastic, and the humanized...
HTP Nanobody Expression Cell-free VHH Synthesis Nanobody humanization Nanobody Structure Nanobodies (VHH) possess the same domains as traditional antibody VH, which includes four conserved framework regions (FR1/2/3/4) and three complementarity-determining regions (CDR1/2/3) (Fig. 2). There are...
Small, single-domain VHH nanobodies, specific for SARS-CoV-2, were proposed for topical use (i.e., by inhalation) as an alternative to systemic mAbs.12,13 However, being nanobodies of camelid origin, they require sophisticated humanization procedures to avoid immunogenic responses, potentially ha...
humanizationin silico developabilitylibrary designNGSsingle domain antibodyVHHyeast surface displayIn this study, we generated a novel library approach for high throughput de novo identification of humanized single-domain antibodies following camelid immunization. To achieve this, VHH-derived complementarity-...
humanizationCX3CR1 has been identified as a highly attractive target for several therapeutic interventions. Despite this potential, no potent antagonists, either small molecule or monoclonal antibody, have been identified. Here we describe the lead finding and engineering approach that lead to the ...