In this study, we have experimentally ablated large projection glutamatergic neurons of the lateral CN and evaluated the impact of this selective manipulation on motor coordination in mice. To this end, through stereotaxic surgery, we injected the lateral CN of Vglut2-Cre mice with an adeno-...
To investigate the role of vGluT2 in CA-RVLM in vivo, we crossed floxed vGluT2 mice with dopamine-尾-hydroxylase (D尾H) Cre mice, creating mice with vGluT2 selectively deleted from D尾H neurons (cKO mice). We then injected a Cre-dependent AAV2 that expresses channelrhodopsin fused to m...
In tests of conditioned place preference, MORflox﹙GluT2cre mice did not acquire place preference for a low dose of the opioid, oxycodone, but displayed conditioned place aversion at a higher dose, whereas control mice displayed preference for both doses. In an oral consumption assessment, these...
In contrast, 螖(9)-tetrahydrocannabinol (螖(9)-THC), the major psychoactive component of cannabis, produced dose-dependent conditioned place aversion and a reduction in the above optical ICSS in VgluT2-cre control mice, but not in VgluT2-CB1 (-/-) mice. These findings suggest that ...
Our data shows that removal of Vglut2 from Trpv1-Cre neurons using transgenic mice abolished both heat and punctuate hyperalgesia associated with nerve injury. In contrast, the development of cold hypersensitivity after nerve injury was unaltered. Here, we show that, VGLUT2-mediated glutamatergic ...
Here we used transgenic mice with Cre recombinase expression driven by the VGluT2 promoter, combined with the chemogenetic approach, to determine the contribution of VGluT2〆xpressing dorsal horn neurons to nociceptive regulation. Adeno゛ssociated viral vectors expressing double‐floxed Creヾependent ...
Conditional knockout of CPEB2 in VGLUT2-expressing glutamatergic neurons impaired consolidation of hippocampus-dependent memory in mice. Presynaptic-specific ablation ofCpeb2in VGLUT2-dominated TA afferents was sufficient to attenuate protein synthesis-dependent LTP. Moreover, blocking activity-induced ...
To test this hypothesis, we used Cre-LoxP techniques to selectively delete VGLUT2 from midbrain DA neurons, and then examined nucleus accumbens (NAc) DA and glutamate responses to METH using in vivo brain microdialysis between DA-VGLUT2-KO mice and their VGLUT2-HET littermates. We found ...
Ai14 mice harbor a loxP-flanked STOP cassette preventing the expression of the fluorescent protein tdTomato. When Ai14 mice were bred with VGLUT2-ires-Cre mice, tdTomato was observed in all VGLUT2+ neurons of the offspring. Four VGAT-EYFP mice (two male and two female) and four VGLUT2...
To assess the role of VGLUT2-mediated glutamatergic transmission in acute and chronic states of pain, we crossed our Vglut2f/f line with the Ht-Pa-Cre line, generating Vglut2f/f;Ht-Pa-Cre mice. This allowed us to associate the role of peripheral VGLUT2-mediated glutamatergic input to ...