In this study, we have experimentally ablated large projection glutamatergic neurons of the lateral CN and evaluated the impact of this selective manipulation on motor coordination in mice. To this end, through stereotaxic surgery, we injected the lateral CN of Vglut2-Cre mice with an adeno-...
To investigate the role of vGluT2 in CA-RVLM in vivo, we crossed floxed vGluT2 mice with dopamine-尾-hydroxylase (D尾H) Cre mice, creating mice with vGluT2 selectively deleted from D尾H neurons (cKO mice). We then injected a Cre-dependent AAV2 that expresses channelrhodopsin fused to m...
Ai14 mice harbor a loxP-flanked STOP cassette preventing the expression of the fluorescent protein tdTomato. When Ai14 mice were bred with VGLUT2-ires-Cre mice, tdTomato was observed in all VGLUT2+ neurons of the offspring. Four VGAT-EYFP mice (two male and two female) and four VGLUT2...
Figure 2. Responses of VGLUT3 lineage neurons to low-intensity fCRD in DSS-treated mice (A) Representative sections through the lumbosacral or coccygeal cord of DSS-treated VGLUT3Cre-tdTomato mice. tdTomato+ neurons were located in laminae II–III (“II–III”), dorsal-lateral funiculus (DLF...
These amacrine cells can be genetically marked in mice where vGluT3 promoter drives expression of a fluorescent reporter or Cre recombinase. It is known that some GCL neurons are also marked. The purpose of the study is to investigate the identity of these GCL neurons. Methods : VgluT3-Cre ...
Here we used transgenic mice with Cre recombinase expression driven by the VGluT2 promoter, combined with the chemogenetic approach, to determine the contribution of VGluT2〆xpressing dorsal horn neurons to nociceptive regulation. Adeno゛ssociated viral vectors expressing double‐floxed Creヾependent ...
In contrast, 螖(9)-tetrahydrocannabinol (螖(9)-THC), the major psychoactive component of cannabis, produced dose-dependent conditioned place aversion and a reduction in the above optical ICSS in VgluT2-cre control mice, but not in VgluT2-CB1 (-/-) mice. These findings suggest that ...
Our data shows that removal of Vglut2 from Trpv1-Cre neurons using transgenic mice abolished both heat and punctuate hyperalgesia associated with nerve injury. In contrast, the development of cold hypersensitivity after nerve injury was unaltered. Here, we show that, VGLUT2-mediated glutamatergic ...
To examine this possibility, we utilized mice expressing Cre-recombinase under control of the endogenous choline acetyltransferase locus and conditionally expressed light-activated Channelrhodopsin2 in CINs. Optical stimulation evoked action potentials in CINs and produced postsynaptic responses in medium spiny...
To assess the role of VGLUT2-mediated glutamatergic transmission in acute and chronic states of pain, we crossed our Vglut2f/f line with the Ht-Pa-Cre line, generating Vglut2f/f;Ht-Pa-Cre mice. This allowed us to associate the role of peripheral VGLUT2-mediated glutamatergic input to ...