energy-dispersive X-ray spectroscopy (EDX) to gain insights into the material composition of the cuticle. We used excitations at six different wavelengths by confocal laser scanning microscopy (CLSM) to gain a more comprehensive understanding of the degree of sclerotization in the forcipular cuticle...
cerevisiae strain, which will result in the protein being expressed as fusion with AGA2 on the outmost cell surface of the yeast cells (Figure 1). Figure 1 The principle of surface display using a-agglutinin system. The allergen of interest (here shown ves v 1) is cloned in-frame with ...
The most common protein families in rattlesnake venoms are: Zn2+2+ metalloproteinases, phospholipases A22s (PLA22s), serine proteinases, C-type lectins (CTLs), disintegrins, L-amino acid oxidase (LAAOs), cysteine rich secretory proteins (CRiSPs), bradykinin potentiating peptides (BPPs), and...
To determine the approximate amount of SVMPs, 15 venoms were separated by RP-HPLC and variation in protein profile and proteolytic activity was evaluated by SDS-PAGE (n = 28) and Hide Powder Azure proteolytic analysis (n = 27). Three types of venom were identified in Mexico which is ...