release of a negative regulatory feedback loop that triggers ERK signaling to protect the cells from apoptotic cell death8,49. The anti-melittin antibody indicated an increasing amount of melittin present in the lysates of both cell lines over time, with a stronger signal for the melittin treatm...
C-type lectin (snaclec), the Ca2+-dependent non-enzymatic proteins9,40of snake venom, are found to be the most abundant non-enzymatic group of proteins in the ECV proteome; however, depending on non-redundant protein / TSA sequence database search their relative occurrence in ECV was demonstr...
The subunits α and β in the heterodimers present sequence homology and are linked by a disulfide bond and loop swapping. Since we found CTLs with masses of ∼16 and 35 kDa and tryptic peptides that assigned for true CTL in the B. jararacussu venom under non-reducing conditions, we ...
(Clemetson, 2010, Arlinghaus and Eble, 2012), and proteoforms of obtustatin (short DISI, 7.4%), a short disintegrin that express the lysine-threonine-serine (KTS) α1β1 integrin inhibitory tripeptide in its active loop (https://www.ncbi.nlm.nih.gov/protein/P83469) (Marcinkiewicz et al...
loop. The most common variant of such catalytically-inactive PLA2homologs is the so-called Lys49 PLA2s, characterized by having a Lys residue in substitution for the canonical Asp at position 49. In addition, these homologs present other substitutions in the calcium-binding loop3,4. ...
The Russell’s viper svPLA2 structure also contains a Trp-31-containing loop (residues 25-34), β-wing consisting of double-stranded anti-parallel β-strands (residues 74-85) and C-terminal region 9 (residues 119-133). The crystal structures of complexes with transition analogs provide ...
The venom and transcriptome profile of the captive Chinese cobra (Naja atra) is not characterized until now. Here, LC-MS/MS and illumine technology were used to unveil the venom and trascriptome of neonates and adults N. atra specimens. In captive Chine
After adenylation of 3 ends of DNA fragments, NEBNext Adaptor with hairpin loop structure was ligated to prepare for hybridization. To select cDNA fragments of preferentially 250–300 bp in length, the library fragments were purified with the AMPure XP system (Beckman Coulter, Beverly, CA, USA...
One hour after injection, edema was evaluated as the percentage increase in weight of the right paw compared to the left one. Antiedematogenic activity was performed by incubating the extracts of sponges (140 mg/kg) with B. jararaca or L. muta venom for 30 min at room temperature, and ...
r. formosensis, also known as the chain snake, is one of the most common causes of snake envenomation. D. r. formosensis venom (DRF proteins) contains a complex of proteins with different biological functions, such as phospholipase A2 (PLA2) [7], activated factor V and hemorrhagins ...