Mice had free access to food, water and were subjected to an artificial light-dark cycle of 12:12 hours. All surgical and experimental procedures used in current study were approved by the IRB committee at Catholic University of Daegu Medical Center (protocol number 2013-1125-CU-AEC-16-Y)....
After hydrolysis of the substrate at the SN-2 position of the thioester bond by the phospholipases, the free thiols generated were detected using 5,5 -dithio-bis-(2-nitrobenzoic acid) (DTNB). The absorbance increase was measured using a spectrophotometer (Bench- mark Plus, Bio-Rad, USA) ...