The spots obtained from the non-reduced gel were previously reduced with 10 mM dithiothreitol/25 mM NH4HCO3 for 1 hour at 56 °C and then alkylated with 55 mM iodoacetamide/25 mM NH4HCO3 for 45 min (reaction took place in the dark). The solution was removed, and the spots were washed ...
filtration peaks (Table1). Further, SVMPs are grouped to PI, PII and PIII classes based on their size and domain structure32. Analysis of LC-MS/MS data against Viperidae venoms showed that ECV was predominated by PIII-SVMPs (17.9% of the ECV proteome) (Table1; Fig.2c). Because SVMP...
normal and cancer cell lines over the hour (two-way ANOVA,p = 0.97). In contrast, melittin significantly reduced the viability of both breast cancer cell lines compared to the normal cells from 10 min onward, and SUM159 significantly more than SKBR3 from 30 min onward (two-way A...
(Clemetson, 2010, Arlinghaus and Eble, 2012), and proteoforms of obtustatin (short DISI, 7.4%), a short disintegrin that express the lysine-threonine-serine (KTS) α1β1 integrin inhibitory tripeptide in its active loop (https://www.ncbi.nlm.nih.gov/protein/P83469) (Marcinkiewicz et al...
After that, 200 μL of blocking buffer, containing 5% bovine serum albumin (BSA) in PBS/Tween20, was added to the wells and the plates were incubated for 1 hour at 37°C. Following this period, the wells were washed and 50 μL of either samples or standard was dispensed into ...
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One hour after injection, edema was evaluated as the percentage increase in weight of the right paw compared to the left one. Antiedematogenic activity was performed by incubating the extracts of sponges (140 mg/kg) with B. jararaca or L. muta venom for 30 min at room temperature, and ...
The entire process, including simulation time, takes roughly one hour for a given ion channel or receptor target. While the results of such an in silico screen may not always be entirely accurate, they can be improved by including mutagenesis constraints from the literature if available. Such ...
Keywords: snake venom metalloproteinases; PII SVMP homologues; disintegrin domain; zinc-binding motif; hemorrhagic activity; platelet aggregation; proteinase activity 1. Introduction Snake venom metalloproteinases (SVMPs) are abundant components in the venoms of advanced snakes of the superfamily Colubro...
After adenylation of 3 ends of DNA fragments, NEBNext Adaptor with hairpin loop structure was ligated to prepare for hybridization. To select cDNA fragments of preferentially 250–300 bp in length, the library fragments were purified with the AMPure XP system (Beckman Coulter, Beverly, CA, USA...