Traces of segmented regions of interest were plotted as ΔF/F0 = (F(t) − F0)/F0, where F0 is defined as the average of all the fluorescence values before the electrical stimulation. The rise time was defined as the time required for the rising phase of the signal to reach...
Considered by some to be among the simpler forms of life, viruses represent highly evolved natural vectors for the transfer of foreign genetic information into cells. This attribute has led to extensive attempts to engineer recombinant viral vectors for
It focuses mainly on development processes and mortality among tick populations so as to provide an overview of how they are regulated in nature and how pathogens can be transmitted under such a framework. The effects of the weather on these life cycles are reviewed. The author also examines ...
Components of excretory/secretory products (ESPs) of helminths have been proposed as vaccine targets and shown to play a role in modulating host immune responses for decades. Such research interest is further increased by the discovery of extracellular vesicles (EVs) in the ESPs of parasitic worm...
Cloning vectors for studies of Caulobacter crescentus genes should be transferable between Escherichia coli and C. crescentus since a transformation system... PV Schoenlein,LM Gallman,B Ely - 《Gene》 被引量: 35发表: 1988年 Filamentous Phages as Cloning Vectors The Ff class of filamentous phages...
As compared to the commonly used vectors, benefits of this design include: (1) minimized non-expressing clones, (2) enhanced stable mAb productivity without gene amplification, (3) control of LC and HC expression at defined ratios, and (4) consistent product quality. After optimization of the...
The sequence to be deleted is cleaved by restriction endonuclease digestion, followed by co-transformation of the linearized plasmid and the oligonucleotide into yeast. By homologous recombination in vivo, a subset of the plasmids will recircularize and simultaneously acquire the deletion as defined by...
Gao et al., Biology of AAV serotype vectors in liver-directed gene transfer to nonhuman primates. Mol Ther. Jan. 2006;13(1):77-87. Epub Oct. 10... G Gao,Y Lu,R Calcedo,... - 《Molecular Therapy》 被引量: 0发表: 2006年 Gene Therapy Vectors Based on Adeno-Associated Virus Type...
Surface modification and genetic engineering are conducted on the vast majority of BNPs, which can be developed as promising vehicles to deliver therapeutic and contrast agents for the imaging, treatment, and prevention of various diseases31–34. Mechanistic studies have contributed invaluable insight ...
REVIEWS Viruses as vaccine vectors for infectious diseases and cancer Simon J. Draper* and Jonathan L. Heeney‡ Abstract | Recent developments in the use of viruses as vaccine vectors have been facilitated by a better understanding of viral biology. Advances occur as we gain greater insight ...