pFLDα vector withsh blegene deletion was amplified using the PCR. The primers used were forward (CYC1HindIII F) and reverse (pTEF1PstI R). The amplified pFLDα without thesh blegene was designated as pFLDα-Z. The pFLDα-Z was amplified using PCR in a 50 μL reaction mixture conta...